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Glycobiology Advance Access published online on March 25, 2009
Glycobiology, doi:10.1093/glycob/cwp048
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© The Author 2009. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

A complex, but uniform O-glycosylation of the human MUC2 mucin from colonic biopsies analyzed by nanoLC/MSn

Jessica M. Holmén Larsson, Hasse Karlsson, Henrik Sjövall and Gunnar C. Hansson

Dept. Medical Biochemistry and Dept. of Medicine, University of Gothenburg, Gothenburg, Sweden

Correspondence to Gunnar C. Hansson, Dept. Medical Biochemistry, P.O. Box 440, 405 30 Gothenburg, Sweden; email: gunnar.hansson{at}medkem.gu.se

Received on March 16, 2009; accepted on March 20, 2009

High sensitivity glycan profiling providing detailed structural information is very important in the search for glycan disease markers. By combining a straight-forward and fast preparation protocol of mucins with high-throughput nanoLC/MS, we have been able to study the O-glycosylation of the colon MUC2 mucin from one single biopsy (~5 mg wet tissue as starting material) collected from the sigmoid colon during routine colonoscopy of 25 normal control patients. This large mucin glycoprotein was recovered from the guanidinium chloride extracted insoluble pellet, reduced and alkylated, separated by SDS-agarose polyacrylamide composite gel electrophoresis and transferred to a PVDF membrane. The O-linked oligosaccharides of the major MUC2 monomer band were released by reductive β-elimination and analyzed by nanoLC/mass spectrometry and MSn. The aim was to identify the MUC2 O-glycans of the sigmoid colon and provide a comprehensive catalogue of the O-glycan repertoire. More than 100 complex O-linked oligosaccharides were identified of which some had not been described before. Most of the oligosaccharides were based on the core 3 structure with sialic acid at the 6-position of the GalNAc and the substructure Galβ1–3/4-GlcNAcβ1–3(NeuAc-6)GalNAcol was found in most glycans. The most abundant components were SO3–-Gal-(Fuc)GlcNAc-3(NeuAc-6)GalNAcol, GalNAc-(NeuAc-)Gal-4/3GlcNAc-3(NeuAc-6)GalNAcol, GalNAc-3(NeuAc-6)GalNAcol, and GlcNAc-3(NeuAc-6)GalNAcol. In contrast to the O-glycans of other mucins, the sigmoid MUC2 O-glycan repertoire and relative amounts in normal individuals were relatively constant.

Key words: Colon / oligosaccharide / glycan / large intestine / mass spectrometry / HPLC


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