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Glycobiology Advance Access published online on November 29, 2008
Glycobiology, doi:10.1093/glycob/cwn136
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© The Author 2008. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Salivary MUC7 is a Major Carrier of Blood Group I Type O-linked Oligosaccharides Serving as the Scaffold for Sialyl Lewis x.

Niclas G. Karlsson1 and Kristina A. Thomsson2

1 National University Ireland, Galway, School of Chemistry, Ireland
2 Department of Medical Biochemistry, Göteborg University, Box 440, 405 30 Göteborg, Sweden

1 To whom correspondence should be addressed; Phone: +353 91 495606, Fax: +353 91 525700, Email: niclas.karlsson{at}nuigalway.ie

Received on October 1, 2008; accepted on November 24, 2008

Isolation of salivary MUC7 with gel electrophoresis allowed analysis by LC-MS and LC-MS2 of released O-linked oligosaccharides and a thorough description of the glycosylation of this molecule, where high molecular weight oligosaccharides up to the size of 2790 Da and with up to three sialic acid residues were identified. A common theme of these novel high abundant oligosaccharides on MUC7 showed that the C-3 branch of the oligosaccharides consisted of branched I-antigen type structural epitopes (GlcNAcβ1–3(GlcNAcβ1–6)Galβ1-), where the branch point was initiated on core 1 and core 2 galactose residues, and the branches were terminated by sialyl type 2 and sialyl Lewis x epitopes. Six sulphated sialylated oligosaccharides of low intensity were also identified, with the sulphate mainly on N-acetyl glucosamine residues located close to the reducing termini. One of these oligosaccharides was identified as a candidate for the high affinity L-selectin ligand 6’ sulpho sialyl Lewis x. Neutral oligosaccharides and blood group antigens were found to be less abundant on MUC7 and the glycosylation appeared to be more preserved between individuals as compared to salivary MUC5B. This was illustrated by comparing LC-MS spectra of MUC7 and MUC5B glycans from secretors (23 individuals) and non-secretors (6 individuals). The data shows that MUC7 provides a multivalent scaffold for sialylation, meeting the requirement for high avidity binding via its glycosylation and mediator of the interaction between immune cells such as salivary neutrophils and oral bacteria.

Key words: Blood group / MG2 / Mucin / Saliva / O-Glycans


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