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Glycobiology Advance Access published online on October 25, 2008
Glycobiology, doi:10.1093/glycob/cwn117
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© The Author 2008. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Complete chemoenzymatic synthesis of the Forssman antigen using novel glycosyltransferases identified in Campylobacter jejuni and Pasteurella multocida

R. Scott Houliston1, Stéphane Bernatchez1, Marie-France Karwaski1, Robert E. Mandrell2, Harold C. Jarrell1, Warren W. Wakarchuk1 and Michel Gilbert1

1 Institute for Biological Sciences, National Research Council Canada, Ottawa, Ontario, Canada, K1A 0R6 US
2 Department of Agriculture, Agricultural Research Service, Produce Safety and Microbiology Research Unit, Albany, CA, USA

Address correspondence to: Michel Gilbert:, Tel: 613-991-9956, Fax: 613-952-9092, email: michel.gilbert{at}nrc-cnrc.gc.ca

Received on July 1, 2008; accepted on October 22, 2008

We have identified an {alpha}1,4-galactosyltransferase (CgtD) and a β1,3-N-acetylgalactosaminyltransferase (CgtE) in the lipooligosaccharide (LOS) locus of Campylobacter jejuni LIO87. Strains that carry these genes may have the capability of synthesizing mimics of the P blood group antigens of the globoseries glycolipids. We have also identified an {alpha}1,3-N-acetylgalactosaminyltransferase (Pm1138) from Pasteurella multocida Pm70, which is involved in the synthesis of an LOS-bound Forssman antigen mimic, and represents the only known bacterial glycosyltransferase with this specificity. The genes encoding the three enzymes were cloned and expressed in Escherichia coli as soluble recombinant proteins that can be used to chemoenzymatically synthesize the Forssman antigen, and its biosynthetic precursors, in high yields.

Key words: Forssman antigen / Campylobacter jejuni / Pasteurella multocida / glycosyltransferase


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