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Glycobiology Advance Access published online on August 19, 2008
Glycobiology, doi:10.1093/glycob/cwn080
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© The Author 2008. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

The Golgi CMP-sialic acid transporter: A new CHO mutant provides functional insights

Sing Fee Lim, May May Lee, Peiqing Zhang and Zhiwei Song

Bioprocessing Technology Institute, Agency for Science, Technology and Research, 20 Biopolis Way, 06-01 Centros, Singapore 138668

Correspondence to: Zhiwei Song, Tel.: +65 6478 8844; Fax: +65 6478 9561; E-mail: song_zhiwei{at}bti.a-star.edu.sg

Received on January 30, 2008; accepted on August 13, 2008

A CHO mutant line, MAR-11, was isolated using a cytotoxic lectin, Maackia amurensis agglutinin (MAA). This mutant has decreased levels of cell surface sialic acid relative to both wild type CHO-K1 and Lec2 mutant CHO cells. The CMP-sialic acid transporter (CMP-SAT) gene in the MAR-11 mutant cell has a C to T mutation that results in a premature stop codon. As a result, MAR-11 cells express a truncated version of CMP-SAT which contains only 100 amino acids rather than the normal CMP-SAT which contains 336 amino acids. Biochemical analyses indicate that recombinant interferon-{gamma} (IFN-{gamma}) produced by the mutant cells lack sialic acid. Using MAR-11 as host cells, an EPO/IEF assay for the structure-function study of CMP-SAT was developed. This assay seems more sensitive than previous assays used to analyze sialylation in Lec2 cells. Co-transfection of constructs that express CMP-SAT into MAR-11 cells completely converted the recombinant EPO to a sialylation pattern that is similar to the EPO produced by the wild type CHO cells. Using this assay, we showed that CMP-SAT lacking C-terminal 18 amino acids from the cytosolic tail was able to allow high levels of EPO sialylation. Substitution of the Gly residues with Ile in 3 different transmembrane domains of CMP-SAT resulted in dramatic decreases in transporter's activity. The CMP-SAT only lost partial activity if the same Gly residues were substituted with Ala, suggesting that the lack of side chain in Gly residues in the transmembrane domains is essential for transport activity.

Key words: CHO cells / CMP-sialic acid transporter / isoelectric focusing (IEF) / recombinant erythropoietin (EPO) / structure-function analysis


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