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Glycobiology Advance Access published online on August 6, 2008
Glycobiology, doi:10.1093/glycob/cwn074
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© The Author 2008. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

The structure and pro-inflammatory activity of the lipopolysaccharide from Burkholderia multivorans and the differences between clonal strains colonizing pre- and post-transplanted lungs

Teresa Ieranòa, Alba Silipoa, Luisa Sturialeb, Domenico Garozzob, Heather Brookesc, C.M. Anjam Khand, Clare Bryantc, F. Kate Goulde,f, Paul A. Corrisf,g, Rosa Lanzettaa, Michelangelo Parrillia, Anthony De Soyzag and Antonio Molinaroa,*

a Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, Napoli, Via Cintia, 4. I-80126 Italy
b Istituto per la Chimica e la Tecnologia dei Materiali Polimerici – ICTMP – CNR, Catania, Italy
c Centre for Veterinary Science, Department of Veterinary Medicine, University of Cambridge, Cambridge, UK
d Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle, UK
e Department of Medical Microbiology
f Cardiopulmonary Transplantation, Freeman Hospital, Newcastle, UK
g Applied Immunobiology and Transplantation Group, Institute of Cellular Medicine, University of Newcastle, Newcastle, UK

* To whom correspondence should be addressed. Phone: +39 081 674123, Fax: +39 081 674393. E-mail: molinaro{at}unina.it

Received on May 30, 2008; accepted on July 29, 2008

The Burkholderia cepacia complex is a group of Gram negative bacteria that are opportunistic pathogens for humans especially in cystic fibrosis patients. Lipopolysaccharide molecules, are potent virulence factors of Gram negative bacteria organisms essential for bacterial survival. A complete analysis of the bacterial lipopolysaccharide structure to function relationship is required to understand the chemical basis of the inflammatory process. We have therefore investigated the structures of lipopolysaccharides from clonally identical Burkholderia multivorans strains (genomovar II) isolated pre- and post- lung transplantation through compositional analysis, mass spectrometry and 2D NMR spectroscopy. We tested the LPS’ pro-inflammatory activity as a stimulant of human myelomonocytic U937 cell cytokine induction and assessed TLR4/MD2 signalling. Marked changes between the paired strains were found in the lipid A-inner core region. Such structural variations can contribute to the bacterial survival and persistence of infections despite the loss of a CF milieu following lung transplantation.

Key words: Burkholderia multivorans / lung transplantation / cystic fibrosis / Lipooligosaccharide / NMR spectroscopy / MS spectrometry


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T. Ierano, A. Silipo, L. Sturiale, D. Garozzo, C. Bryant, R. Lanzetta, M. Parrilli, C. Aldridge, F K. Gould, P. A Corris, et al.
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[Abstract] [Full Text] [PDF]


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