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Glycobiology Advance Access published online on February 6, 2008
Glycobiology, doi:10.1093/glycob/cwn010
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© The Author 2008. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Differentiating N-linked Glycan Structural Isomers in Metastatic and Non- Metastatic Tumor Cells using Sequential Mass Spectrometry

Justin M. Prien{dagger}, Leanne C. Huysentruyt*, David J. Ashline{dagger}, Anthony J. Lapadula{dagger},{ddagger},, Thomas N. Seyfried* and Vernon N. Reinhold{dagger}

{dagger} The Glycomics Center, Division of Molecular, Cellular, and Biomedical Sciences
{ddagger} Department of Computer Science, University of New Hampshire, Durham, NH 03824
* Department of Biology, Boston College, Chestnut Hill, Massachusetts 02467

Received on January 13, 2008; accepted on February 1, 2008

A murine cell line model that manifests all the hallmarks of metastatic disease was used to demonstrate the capability of ion trap sequential mass spectrometry (MSn) methodology for detailed glycan structural characterization and its potential for glycan biomarker discovery. Differential glycome profiles identified modulated ions which were selected for a detailed investigation. From these data multiple cancer cell line specific glycan structures were identified. These unique structures were characterized within isomeric compositions as pendant non–reducing termini of di-, and trisaccharide fragments, distinctively transparent to sequential ion trap MSn detection.

Key words: carbohydrate / oligosaccharide / malignancy / biomarker / isomer


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