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Glycobiology Advance Access published online on October 27, 2007
Glycobiology, doi:10.1093/glycob/cwm116
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© The Author 2007. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Characterization and Protection on Acute Liver Injury of Polysaccharide MP-I from Mytilus Coruscus

Hongli Xua,*, Tingting Guob, Yi-feng Guoa, Jianpeng Zhanga, Yang Lia, Weihua Fenga,* and Binghua Jiaoa,*

a Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, PR China
b Central Laboratory, Aqua-life Science and Technology College, Shanghai Fisheries University, Shanghai 200090, PR China

* Correspondance about the manuscript should be addressed to Hongli Xu: Fax: 0086-021-65334344; Phone: 0086-021-25070306-8007; e-mail address: beam1981{at}21cn.com

* Corresponding author: Weihua Feng Fax: 0086-021-65334344 Phone: 0086-021-25074233 E-mail address: fwh402{at}163.com

* Corresponding author:Binghua Jiao Fax: 0086-021-65334344 Phone: 0086-021-25070306-8001 E-mail address: jiaobh{at}uninet.cn

Received on August 7, 2007; accepted on October 12, 2007

In this study, we analyzed a water-soluble polysaccharide MP-I isolated from Mytilus coruscus. MP-I was obtained by hot-water extraction, anion-exchange and gel-permeation chromatography. Complete hydrolysis, periodate oxidation, methylation analysis, as well as fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) spectroscopy were conducted to elucidate its structure. MP-I was subjected to investigate the protective effect on carbon tetrachloride (CCl4) induced liver damage in male Kunming mice. Based on the data obtained, MP-I was found to be an {alpha}-(1->4)-D-glucan, branched with a single {alpha}-D-glucose at the C-6 position every eight residue, on average, along the main chain. Based on the calibration with dextran, the glucan had a molecular weight of about 1.35x106 Da. Pharmacological studies revealed that MP-I could decrease serum alanine aminotransferase (ALT), serum aspartate aminotransferase (AST) and hepatic malondialdehyde aldehydes (MDA) levels, increase hepatic total superoxide dismutase (T-SOD) activity and improve hepatic damage in the CCl4 induced liver injury in mice in a dose-dependent manner. The results suggest that the possible mechanism is due to it's antioxidant activity of MP-I.

Key words: antioxidant / carbon tetrachloride / liver injury / mytilus coruscus polysaccharide I / structure


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