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Glycobiology Advance Access published online on June 29, 2007
Glycobiology, doi:10.1093/glycob/cwm066
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© The Author 2007. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Characterization and role of fucose mutarotase in mammalian cell

Dongkyu Park*, Kyoung-Seok Ryu{dagger}, Dongwook Choi*, Jaechan Kwak* and Chankyu Park*,

* Department of Life Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-Dong, Yuseong-Gu, Daejeon 305-701, Korea
{dagger} Magnetic Resonance Team, Korea Basic Science Institute, 52 Yeoeun-Dong, Yuseong-Gu, Daejeon 305-333, Korea

Address correspondence to: Chankyu Park, Department of Life Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-Dong, Yuseong-Gu, Daejeon 305-701, Korea, Tel: 82-42-869-2629; Fax: 82-42-869-2610; E-mail: ckpark{at}kaist.ac.kr

Received on April 19, 2007; accepted on June 15, 2007

L-fucose for mammalian glycosylation contains an anomeric carbon atom generating {alpha}- and ß-L-fucoses. Based on sequence comparison of mouse and human homologs with the procaryotic fucose mutarotases (FucU) characterized previously (Ryu et al., (2004) J. Biol. Chem. 279, 25544-25548), we investigated their function in mammalian cells. By NMR measurement with saturation difference analysis, the purified mammalian mutarotases were demonstrated to be involved in an interconversion between the two anomeric forms with comparable efficiency as that of the E. coli FucU. The mouse gene was widely expressed in various tissues and cell lines, including kidney, liver, and pancreas, although expression was marginal in muscle and testis. By generating stably expressed cell lines for mutarotase genes in HepG2, it was shown that fucose incorporations into cellular proteins were increased as demonstrated by an incorporation of radiolabeled fucose into the cells. Furthermore, intracellular levels of GDP-L-fucose, measured with HPLC, were enhanced by an overproduction of cellular mutarotase, which was reversed by gene silencing of mutarotase based on RNA interference. The results suggest that the mammalian mutarotase is functional in facilitated incorporation of fucose through the salvage pathway.

Key words: Fucose / Mutarotase / NMR / Fucose incorporation / HPLC


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