Glycobiology Advance Access published online on April 3, 2007
Glycobiology, doi:10.1093/glycob/cwm039
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Enzymatic synthesis of cello-oligosaccharides by rice BGlu1 ß-glucosidase glycosynthase mutants
1 Department of Biochemistry and Center for Protein Structure and Function, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
2 Protein Engineering Network of Centres of Excellence and Department of Chemistry, University of British Columbia, Vancouver, B.C., Canada, V6T 1Z1
3 Institute of Science, School of Biochemistry, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
* Corresponding author: Jisnuson Svasti, Department of Biochemistry and Center for Protein Structure and Function,Faculty of Science, Mahidol University, Bangkok 10400, Thailand Telephone: +66 02 2015845 Fax: +66 02 2015843 E-mail: scjsv{at}mahidol.ac.th
Received on November 29, 2006; revised on March 20, 2007; accepted on March 25, 2007
Rice (BGlu1) ß-glucosidase is a glycosyl hydrolase family 1 enzyme that acts as an exoglucanase on ß-(1,4)- and short ß-(1,3)-linked gluco-oligosaccharides. Mutations of BGlu1 ß-glucosidase at glutamate residue 414 of its natural precursor destroyed the enzyme's catalytic activity, but the enzyme could be rescued in the presence of the anionic nucleophiles formate and azide, which verifies that this residue is the catalytic nucleophile. The catalytic activities of three candidate mutants, E414G, E414S, and E414A, in the presence of the nucleophiles were compared. The E414G mutant had approximately 25- and 1400-fold higher catalytic efficiency than E414A and E414S, respectively. All three mutants could catalyze the synthesis of mixed length oligosaccharides by transglucosylation, when 
-glucosyl fluoride was used as donor and pNP-cellobioside as acceptor. The E414G mutant gave the fastest transglucosylation rate, which was approximately 3 and 19 fold faster than E414S and E414A, respectively, and gave yields of up to 70-80 % insoluble products with a donor: acceptor ratio of 5:1. 13C-NMR, methylation analysis and electrospray ionization mass spectrometry showed that the insoluble products were ß-(1,4)-linked oligomers with a degree of polymerization (DP) of 5 to at least 11. The BGlu1 E414G glycosynthase was found to prefer longer chain length oligosaccharides that occupy at least three sugar residue binding subsites as acceptors for productive transglucosylation. This is the first report of a ß-glucansynthase derived from an exoglycosidase that can produce long chain cello-oligosaccharides, which likely reflects the extended oligosaccharide binding site of rice BGlu1 ß-glucosidase.
Key words: ß-glucosidase / glycosynthase / oligosaccharide synthesis / rice / transglucosylation
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