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Glycobiology Advance Access published online on March 6, 2007
Glycobiology, doi:10.1093/glycob/cwm024
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© The Author 2007. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Integrated mass spectrometric strategy for characterising the glycans from glycosphingolipids and glycoproteins : direct identification of sialyl Lex in mouse

Simon Parry1, Victoria Ledger1, Bérangère Tissot1, Stuart M. Haslam1, James Scott2, Howard R. Morris1,3 and Anne Dell1,*

1 Division of Molecular Biosciences, Imperial College London, London SW7 2AZ, UK
2 Imperial College Genetics & Genomics Research Institute, London SW7 2AZ, UK
3 M-SCAN Ltd., Wokingham, Berks RG41 2TZ, UK

* corresponding author: phone: 44-207-5945219, fax: 44-207-2250458, e-mail: a.dell{at}imperial.ac.uk

Received on December 14, 2006; revised on February 21, 2007; accepted on February 22, 2007

The current interest in applying systems biology approaches to studying an organism's form or function promises to reveal further insights into the role of glycosylation in cells and whole organisms. This has prompted the development of a rapid, sensitive method of profiling the glycan component of both glycosphingolipids and glycoproteins from a single sample. Here we report a new mass spectrometric screening strategy for characterising glycosphingolipid-derived oligosaccharides which can be integrated into an existing highly sensitive glycoprotein glycomics strategy. Using ceramide glycanase to release the glycans from glycosphingolipids, this method provides a reliable profile of the glycosphingolipid-derived glycans present in a sample and has revealed new glycan structures. Glycoproteins are also efficiently recovered using this method allowing the subsequent analysis of glycoprotein-derived glycans by mass spectrometry. The high sensitivity of this glycomic screening method allowed us to directly characterise the sialyl Lex epitope from mouse brain for the first time, where it was observed on an O-mannose structure. Thus, we present a mass spectrometric method that allows glycomic screening of N- and O-glycans as well as glycosphingolipid-derived glycans from a single tissue.

Key words: glycan / glycosphingolipid / glycoprotein / mass spectrometry / method


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