Glycobiology Advance Access published online on January 3, 2007
Glycobiology, doi:10.1093/glycob/cwl083
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Virus-induced transcriptional activation of host FUTgenes associated with neo-expression of Ley in cytomegalovirus- and sialyl-Lex in varicella-zoster virus-infected diploid human cells
1 Department of Virology, University of Göteborg, Göteborg
2 Department of Clinical Chemistry and Transfusion Medicine, University of Göteborg, Göteborg
3 Department of Virology, Swedish Institute for Infectious Disease Control, Solna, Sweden
4 Dental School, University of Copenhagen, Copenhagen, Denmark
* Corresponding author Dr. Sigvard Olofsson Department of Virology, University of Göteborg, Guldhedsgatan 10B, S-413 46 Göteborg, Sweden Phone +46 31 342 46 59, Fax +46 31 82 70 32, sigvard.olofsson{at}microbio.gu.se
Received on March 21, 2006; revised on December 19, 2006; accepted on December 21, 2006
Cell surface carbohydrate structures including sialyl Lewis X (sLex) and Lewis Y (Ley) are engaged in important recognition phenomena in normal and malignant tissue. The aim here was to determine possible influence on the expression of such antigens by two viruses, varicella-zoster virus (VZV) and cytomegalovirus (CMV), involved in persistent infections of humans. We found that infection of human diploid fibroblasts with both viruses resulted in transcriptional activation of several fucosyltransferase genes that were either dormant or expressed at low levels in uninfected cells. Both viruses induced FUT3, FUT5and FUT6, encoding 
1,3 and or 1,4-specific fucosyltransferases. CMV, but not VZV induced transcription of FUT1(encoding an 1,2-specific fucosyltransferase), FUT7, and FUT9. The changes in transcription of fucosyltransferase genes were expectedly associated with expression of Ley in CMV-infected cells and sLex in the VZV-infected fibroblasts although no expression of these antigens was observed in uninfected cells. One major explanation to this difference between CMV-and VZV-infected cells, was that CMV but not VZV induced expression of FUT1, necessary for Ley expression. The induced carbohydrate antigens in CMV- and VZV-infected cells could be of significance for virus spread and possible decoying of immune responses.
Key words: cytomegalovirus / fucosyltransferase / Lewis Y / sialyl-Lewis X / varicella-zoster virus
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