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Glycobiology Advance Access published online on September 15, 2006
Glycobiology, doi:10.1093/glycob/cwl050
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© The Author 2006. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Received July 12, 2006
Revised September 8, 2006
Accepted September 11, 2006

Article

Major O-glycans in the spores of two microsporidian parasites are represented by unbranched manno-oligosaccharides containing {alpha}-1,2 linkages

Vanessa Taupin 1, Estelle Garenaux 2, Muriel Mazet 1, Emmanuel Maes 2, Hubert Denise 1, Gérard Prensier 1, Christian P. Vivarès 1 *, Yann Guérardel 2, and Guy Méténier 1

1 Equipe Parasitologie Moléculaire et Cellulaire, LBP, CNRS UMR6023, Université Blaise Pascal, 24 Avenue des Landais, 63177 Aubière Cedex, France
2 Laboratoire de Glycobiologie Structurale et Fonctionnelle, CNRS UMR8576, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d'Ascq Cedex, France

* To whom correspondence should be addressed.
Christian P. Vivarès, E-mail: christian.vivares{at}univ-bpclermont.fr


  Abstract

Protein glycosylation in microsporidia, a fungi-related group comprising exclusively obligate intracellular parasitic species, is still poorly documented. Here, we have studied glycoconjugate localization and glycan structures in spores of Encephalitozoon cuniculi and Antonospora locustae, two distantly related microsporidians invading mammalian and insect hosts, respectively. The polar sac-anchoring disc complex or polar cap, an apical element of the sporal invasion apparatus, was strongly PATAg-positive. Mannose-binding lectins reacted with the polar cap and recognized several bands (from 20 to 160 kDa) on blots of E. cuniculi protein extracts. Physicochemical analyses provided the first determination of major glycostructures in microsporidia. O-linked glycans were demonstrated to be linear manno-oligosaccharides containing up to eight {alpha}1,2-linked mannose residues, thus resembling those reported in some fungi such as Candida albicans. No N-linked glycans were detected. The data are in accordance with gene-based prediction of a minimal O-mannosylation pathway. Further identification of individual mannoproteins should help in the understanding of spore germination mechanism and host-microsporidia interactions.

Keywords: glycan analysis; microsporidia; O-mannosylation; polar cap; ultracytochemistry.
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