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Glycobiology Advance Access published online on September 13, 2006
Glycobiology, doi:10.1093/glycob/cwl048
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© The Author 2006. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Received July 19, 2006
Revised September 1, 2006
Accepted September 6, 2006

Article

Structural characterization of N-glycans from the freshwater snail Biomphalaria glabrata cross-reacting with Schistosoma mansoni glycoconjugates

Tobias Lehr 1, Hildegard Geyer 1, Kai Maaß 1, Michael J. Doenhoff 2, and Rudolf Geyer 1 *

1 Institute of Biochemistry, Faculty of Medicine, University of Giessen, Friedrichstrasse 24 D-35392 Giessen, Germany
2 School of Biological Sciences, University of Wales, Bangor, Gwynedd LL57 2UW, Wales U.K.

* To whom correspondence should be addressed.
Rudolf Geyer, E-mail: Rudolf.Geyer{at}biochemie.med.uni-giessen.de


  Abstract

The human parasitic trematode Schistosoma mansoni has a complex life-cycle which includes the freshwater snail Biomphalaria glabrata as intermediate host. Within each stage, the parasite synthesizes a wide array of glycoconjugates exhibiting, in part, unique carbohydrate structures. In addition, the parasite expresses definitive host-like sugar epitopes, such as Lewis X determinants, supporting the concept of carbohydrate-mediated molecular mimicry as an invasion and survival strategy. In the present study we investigated whether common carbohydrate determinants occur also at the level of the intermediate host. To this end, a structural characterization of haemolymph glycoprotein-N-glycans of B. glabrata was performed.

N-glycans were released from tryptic glycopeptides and labelled with 2-aminopyridine. Sugar chains serologically cross-reacting with S. mansoni glycoconjugates were isolated by immunoaffinity chromatography using a polyclonal antiserum directed against schistosomal egg antigens and fractionated by Aleuria aurantia lectin affinity chromatography and high-performance liquid chromatography. Obtained glycans were analyzed by different mass spectrometric techniques as well as monosaccharide constituent and linkage analysis. The results revealed a highly heterogeneous oligosaccharide pattern. Cross-reacting species represented about 5 % of the total glycans and exhibited either a terminal Fuc({alpha}1-3)GalNAc-unit, a (1-2)-linked xylosyl residue or both types of structural motifs. In conclusion, our study demonstrates the presence of common carbohydrate epitopes also at the level of S. mansoni and its intermediate host.

Keywords: Biomphalaria glabrata; mass spectrometry; mollusc glycoproteins; N-glycans; Schistosoma.
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