Glycobiology Advance Access published online on September 14, 2006
Glycobiology, doi:10.1093/glycob/cwl047
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1 Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Central-2, Open Space Laboratory, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8568, Japan,; Department of Anatomy and Embryology, Biomolecular and Integrated Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan
* To whom correspondence should be addressed. The 3-fucosyl-N-acetyllactosamine (Lewis x (Lex), CD15, SSEA-1) carbohydrate structure is expressed on several glycolipids, glycoproteins, and proteoglycans of the nervous system and has been implicated in cell-cell recognition, neurite outgrowth, and neuronal migration during development. To characterize the functional role of Lex carbohydrate structure in vivo, we have generated mutant mice that lack
Received February 8, 2006
Revised August 26, 2006
Accepted September 5, 2006
Article
Mice lacking
Takashi Kudo 1, Takashi Fujii 2, Shiro Ikegami 3, Kaoru Inokuchi 3, Yuko Takayama 2, Yuzuru Ikehara 4, Shoko Nishihara 5, Akira Togayachi 2, Satoru Takahashi 6, Kouichi Tachibana 2, Shigeki Yuasa 7, and Hisashi Narimatsu 8 *
1,3-fucosyltransferase IX demonstrate disappearance of Lewis x structure in brain and increased anxiety-like behaviors
2 Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Central-2, Open Space Laboratory, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8568, Japan
3 Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan
4 Division of Oncological Pathology, Aichi Cancer Centre Research Institute, Chikusa-ku, Nagoya, Aichi 464-0021, Japan
5 Laboratory of Cell Biology, Department of Bioinformatics, Faculty of Engineering, Soka University, 1-236 Tangi-cho, Hachioji, Tokyo 192-8577, Japan
6 Department of Anatomy and Embryology, Biomolecular and Integrated Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan
7 Department of Ultrastructural Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan
8 Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Central-2, Open Space Laboratory, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan; Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Central-2, Open Space Laboratory, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8568, Japan
Hisashi Narimatsu, E-mail: h.narimatsu{at}aist.go.jp
Abstract 
1,3-fucosyltransferase IX (Fut9-/-). Fut9-/- mice were unable to synthesize the Lex structure carried on glycoproteins and glycolipids in embryonic and adult brain. However, no obvious pathological differences between wild-type and Fut9-/- mice were found in brain. In behavioral tests, Fut9-/- mice exhibited increased anxiety-like responses in dark-light preference and in elevated + maze tests. Immunohistochemical analysis showed that the number of calbindin-positive neurons was decreased in the basolateral amygdala in Fut9-/- mice. These observations indicated that the carbohydrates synthesized by Fut9 play critical roles in functional regulations of interneurons in the amygdalar subdivisions and suggested a role for the Lex structure in some aspects of emotional behavior in mice.1,3-fucosyltransferase; Lewis x; knockout mouse; anxiety; amygdala.
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