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Glycobiology Advance Access published online on August 17, 2006
Glycobiology, doi:10.1093/glycob/cwl037
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© The Author 2006. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Received June 2, 2006
Revised July 12, 2006
Accepted August 4, 2006

Article

Effects of N-glycan processing inhibitors on signaling events and induction of apoptosis in galectin-1 stimulated Jurkat T lymphocytes

Hermann Walzel 1 *, Abdelgawad A. Fahmi 2, Mohamed A. Eldesouky 2, Ehab F. Abou-Eladab 3, Grit Waitz 1, Josef Brock 1, and Markus Tiedge 1

1 Institute of Medical Biochemistry and Molecular Biology, University of Rostock, Schillingallee 70, D-18057 Rostock, Germany
2 Cairo University, Faculty of Science, Chemistry Department, Egypt
3 Mansoura University, Faculty of Specific Education, New Damietta City, Egypt

* To whom correspondence should be addressed.
Hermann Walzel, E-mail: hermann.walzel{at}med.uni-rostock.de


  Abstract

To elucidate the role of N-linked glycans in triggering T cell functions the effects of the N-glycan processing inhibitors 1-deoxymannojirimycin (1-DMM) and swainsonine (SW) were investigated on signaling events and induction of apoptosis in galectin-1 (gal-1) stimulated Jurkat T-lymphocytes. Treatment of Jurkat E6.1 cells with 1-DMM and SW strongly reduced the cell binding of gal-1-biotin, conjugate binding to cell lysate glycoproteins, and to CD3-immunoprecipitates on blots as well as the binding of CD2 and CD3 to immobilized gal-1. The mannosidase inhibitors efficiently decreased gal-1 induced calcium mobilization. Both phases originated from a transient Ca2+-release of internal stores and the sustained influx across the plasma membrane were found to be involved. Both inhibitors suppressed in transiently transfected Jurkat T-lymphocytes the gal-1 induced expression of the luciferase reporter gene constructs pNFAT-TA-Luc and pAP1(PMA)-TA-Luc. The data provide evidence that gal-1 triggers through binding to N-linked glycans a Ca2+-sensitive apoptotic pathway.

Keywords: apoptosis/galectin-1/Jurkat T-lymphocytes/N-glycan processing/signaling.
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