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Glycobiology Advance Access published online on July 28, 2006
Glycobiology, doi:10.1093/glycob/cwl033
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© The Author 2006. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Received June 15, 2006
Revised July 26, 2006
Accepted July 27, 2006

Article

Core oligosaccharide structure from the highly phytopathogenic A. tumefaciens TT111 and conformational analysis of the putative Rhamnan epitope

Cristina De Castro 1 *, Anna Carannante 1, Rosa Lanzetta 1, Valeria Liparoti 1, Antonio Molinaro 1, and Michelangelo Parrilli 1

1 Department of Organic Chemistry and Biochemistry, University of Naples, Complesso Universitario Monte Sant’ Angelo, Via Cintia 4, 80126 Napoli, Italy

* To whom correspondence should be addressed.
Cristina De Castro, E-mail: decastro{at}unina.it


  Abstract

The structure of the complex mixture of the core oligosaccharide components of the lipooligosaccharide fraction of Agrobacterium tumefaciens strain TT111 was determined directly on the deacetylated products, by means of spectroscopical methods. The Rhamnan oligosaccharide elongating the inner Kdo residue shares structural features with other polysaccharide from well-known plant pathogenic bacteria. Its conformation was determined thorough extensive Molecular Dynamic analysis and anneals forming an epitope similar to that recognised from the plant defence system.

Keywords: conformation analysis/Lipooligosaccharide/NMR spectroscopy/structure elucidation.
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