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Glycobiology Advance Access published online on July 27, 2006
Glycobiology, doi:10.1093/glycob/cwl027
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© The Author 2006. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Received March 16, 2006
Revised July 21, 2006
Accepted July 23, 2006

Article

Down-regulation of {beta}1,4-galactosyltransferase V is a critical part of etoposide-induced apoptotic process and could be mediated by decreasing Sp1 levels in human glioma cells

Jianhai Jiang 1, Jialin Shen 1, Yuanyan Wei 1, Tao Wu 1, Xiaoning Chen 1, Hongliang Zong 1, Si Zhang 1, Maoyun Sun 1, Jianhui Xie 1, Xiangfei Kong 1, Yanzhong Yang 1, Aiguo Shen 1, Hanzhou Wang 1, and Jianxin Gu 1 *

1 Key Laboratory of Medical Molecular Virology Ministry of Education and Health, Gene Research Center, Shanghai Medical College of Fudan University (former Shanghai Medical University), Shanghai 200032, China

* To whom correspondence should be addressed.
Jianxin Gu, E-mail: jxgu{at}shmu.edu.cn


  Abstract

{beta}1,4-Galactosyltransferase V ({beta}1,4GalT V; EC 2.4.1.38) is considered to be very important in glioma for expressing transformation-related highly branched N-glycans. Recently, we have characterized {beta}1,4GalT V as a positive growth regulator in several glioma cell lines. However, the role of {beta}1,4GalT V in glioma therapy has not been clearly reported. In this study, interfering with the expression of {beta}1,4GalT V by its antisense cDNA in SHG44 human glioma cells markedly promoted apoptosis induced by etoposide and activation of caspases as well as processing of Bid and expression of Bax and Bak. Conversely, the ectopic expression of {beta}1,4GalT V attenuated the apoptotic effect of etoposide on SHG44 cells. In addition, both the {beta}1,4GalT V transcription and the binding of total or membrane glycoprotein with RCA-I were partially reduced in etoposide-treated SHG44 cells, correlated well with a decreased level of Sp1 which has been identified as an activator of {beta}1,4GalT V transcription. Collectively, our results suggest that down-regulation of {beta}1,4GalT V expression plays an important role in etoposide-induced apoptosis and could be mediated by a decreasing level of Sp1 in SHG44 cells, indicating that inhibitors of {beta}1,4GalT V may enhance the therapeutic efficiency of etoposide for malignant glioma.

Keywords: {beta}1,4-galactosyltransferase V/apoptosis/etoposide/glioma/Sp1.
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J. Zhou, Y. Wei, D. Liu, X. Ge, F. Zhou, X. Yun, J. Jiang, and J. Gu
Identification of {beta}1,4GalT II as a Target Gene of p53-mediated HeLa Cell Apoptosis
J. Biochem., April 1, 2008; 143(4): 547 - 554.
[Abstract] [Full Text] [PDF]


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