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Glycobiology Advance Access published online on May 2, 2006
Glycobiology, doi:10.1093/glycob/cwj126
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© 2006 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited
Received January 20, 2006
Revised April 20, 2006
Accepted April 28, 2006

Article

Two categories of mammalian galactose-binding receptors distinguished by glycan array profiling

Peter J. Coombs 1, Maureen E. Taylor 2, and Kurt Drickamer 2 *

1 Division of Molecular Biosciences, Imperial College, London, SW7 2AZ, UK; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
2 Division of Molecular Biosciences, Imperial College, London, SW7 2AZ, UK

* To whom correspondence should be addressed.
Kurt Drickamer, E-mail: k.drickamer{at}imperial.ac.uk


  Abstract

Profiling of the four known galactose-binding receptors in the C-type lectin family has been undertaken in parallel on a glycan array. The results are generally consistent with previous assays using various different formats, but they provide a direct comparison of the properties of the four receptors, revealing that they fall into two distinct groups. The major subunit of the rat asialoglycoprotein receptor and the rat Kupffer cell receptor show similar broad preference for GalNAc-terminated glycans, while the rat macrophage galactose lectin and the human scavenger receptor C-type lectin bind more restricted sets of glycans. Both of these receptors bind to Lewisx-type structures, but the macrophage galactose lectin also interacts strongly with bi-antennary galactose- and GalNAc-terminated glycans. Although the similar glycan-binding profiles for the asialoglycoprotein receptor and the Kupffer cell receptor might suggest that these receptors are functionally redundant, analysis of fibroblasts transfected with full-length Kupffer cell receptor reveals that they fail to endocytose glycosylated ligand.

Keywords: glycan array/lectin/Kupffer cell receptor/asialoglycoprotein receptor/scavenger receptor.
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