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Glycobiology Advance Access published online on May 5, 2006
Glycobiology, doi:10.1093/glycob/cwj124
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© The Author 2006. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Received February 3, 2006
Revised April 12, 2006
Accepted April 26, 2006

Article

Molecular evolution of protein O-fucosyltransferase genes and splice variants

C. Loriol 1, F. Dupuy 1, R. Rampal 2, M.A. Dlugosz 2, R.S. Haltiwanger 2, A. Maftah 1 *, and A. Germot 1

1 INRA, UMR 1061, Unité de Génétique Moléculaire Animale; GDR-CNRS 2590; Université de Limoges, Institut des Sciences de la Vie et de la Santé, Faculté des Sciences et Techniques, 87060 Limoges, France
2 Department of Biochemistry and Cell Biology, Institute for Cell and Developmental Biology, State University of New York, Stony Brook, New York, USA

* To whom correspondence should be addressed.
A. Maftah, E-mail: maftah{at}unilim.fr


  Abstract

O-Fucose has been described on both epidermal growth factor-like (EGF) repeats and Thrombospondin type 1 repeats (TSR). The enzyme adding fucose to EGF repeats, protein O-fucosyltransferase 1 (Pofut1), is a soluble protein located in the lumen of endoplasmic reticulum. A second protein O-fucosyltransferase, Pofut2, quite divergent from its homologue Pofut1, has recently been shown to O-fucosylate TSRs but not EGF repeats. To date Pofut1 genes have only been characterized in human, mouse and fly, and Pofut2 in mouse, fly, and partially in the nematode Caenorhabditis elegans. Here we report cDNA sequences and genomic structures of bovine Pofut1 and Pofut2 genes and describe for the first time five alternative spliced transcripts for each gene. Only one transcript for both Pofut1 and Pofut2 encodes an active bovine O-fucosyltransferase. Variant transcript distribution was examined in thirteen bovine tissues. Transcripts encoding active forms are ubiquitous while other forms possess a more restricted tissue-expression profile. Sequence comparison and phylogenetic analyses revealed that both Pofut genes are present as a single copy in animal genomes, and their exon-intron organizations are conserved among vertebrates. The last common ancestor of all analyzed bilaterian species would be predicted to possess polyexonic Pofut genes in their genome.

Keywords: Alternative splicing/Fucose/Gene organization/O-fucosyltransferase activity/Pofut1 and Pofut2 phylogeny.
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