Glycobiology Advance Access published online on April 3, 2006
Glycobiology, doi:10.1093/glycob/cwj110
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1 Division of Molecular Biosciences, Imperial College London, South Kensington, London SW7 2AZ, UK
* To whom correspondence should be addressed. The MUC1 mucin is an important tumor-associated antigen that shows extensive glycosylation in vivo. The O-glycosylation of this molecule, which has been well characterized in a number of cell types and tissues, is important in conferring the unusual biochemical and biophysical properties on a mucin. N-glycosylation is crucial to the folding, sorting, membrane trafficking and secretion of many proteins. Here we evaluated the N-glycosylation of MUC1 derived from two sources: endogenous MUC1 isolated from human milk and a recombinant epitope-tagged MUC1F overexpressed in Caco2 colon carcinoma cells . N-glycans on purified MUC1F/MUC1 were analysed by MALDI-TOF-MS, GC-MS and CAD-ESI-MS/MS. The spectra indicate that MUC1F N-glycans have MUC1F N-glycans have compositions consistent with high mannose structures (Hex5-9HexNAc2) and complex / hybrid type glycans (NeuAc0-3Fuc0-3Hex3-8HexNAc3-7). Many of the Nglycan structures are identical on MUC1F and native MUC1, however, a marked difference is seen between the N-glycans on membrane-bound and secreted forms of the native molecule.
Received December 14, 2005
Revised February 28, 2006
Accepted March 26, 2006
Article
N-glycosylation of the MUC1 mucin in epithelial cells and secretions
Simon Parry 1,
Franz Georg Hanisch 2,
Shih-Hsing Leir 3,
Mark Sutton-Smith 1,
Howard R. Morris 4,
Anne Dell 1,
and
Ann Harris 5 *
2 Center of Biochemistry, Medical Faculty, and Center for Molecular Cologne, University of Cologne Joseph-Stelzmann-Str. 52, 50931 Köln, Germany
3 Paediatric Molecular Genetics, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS, UK
4 Division of Molecular Biosciences, Imperial College London, South Kensington, London SW7 2AZ, UK; M-SCAN Mass Spectrometry Research and Training Centre, Silwood Park, Ascot SL5 7PZ, UK
5 Paediatric Molecular Genetics, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS, UK; Current address: Human Molecular Genetics Program, Children’s Memorial Research Center, Northwestern University, 2300 Children’s Plaza, Box 211, Chicago. Il 60614-3394 US
Ann Harris, E-mail: ann-harris{at}northwestern.edu
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