Skip Navigation



Glycobiology Advance Access published online on March 10, 2006
Glycobiology, doi:10.1093/glycob/cwj100
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrowOA All Versions of this Article:
16/6/564    most recent
cwj100v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Shigeta, M.
Right arrow Articles by Gu, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shigeta, M.
Right arrow Articles by Gu, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published by Oxford University Press 2006. All rights reserved. The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org
Received January 13, 2006
Revised February 27, 2006
Accepted March 2, 2006

Article

{beta}1,4-N-Acetylglucosaminyltransferase III potentiates {beta}1 integrin-mediated neuritogenesis induced by serum deprivation in Neuro2a cells

Masaki Shigeta 1, Yukinao Shibukawa 1, Hideyuki Ihara 1, Eiji Miyoshi 1, Naoyuki Taniguchi 1 *, and Jianguo Gu 2 *

1 Department of Biochemistry, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
2 Department of Biochemistry, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan; Division of Regulatory Glycobiology, Tohoku Pharmaceutical University, 4-4-1 Komatsusima, Aobaku, Sendai, Miyagi 981-8558, Japan

* To whom correspondence should be addressed.
Naoyuki Taniguchi, E-mail: proftani{at}biochem.med.osaka-u.ac.jp
Jianguo Gu, E-mail: jgu{at}biochem.med.osaka-u.ac.jp


  Abstract

Aspects of the biological significance of the bisecting N-acetylglucosamine (GlcNAc) structure on N-glycans introduced by {beta}1,4-N-acetylglucosaminyltransferase III (GnT-III) in Neuro2a cell differentiation is demonstrated. The overexpression of GnT-III in the cells led to the induction of axon-like processes with numerous neurites and swellings, in which {beta}1 integrin was localized, under conditions of serum starvation. This enhancement in neuritogenesis was suppressed by either the addition of a bisecting GlcNAc-containing N-glycan or E4-PHA, which preferentially recognizes the bisecting GlcNAc. GnT-III-promoted neuritogenesis was also significantly perturbed by treatment with a functional blocking anti-{beta}1 integrin antibody. In fact, {beta}1 integrin was found to be one of target proteins of GnT-III, as confirmed by a pull down assay with E4-PHA. These data suggest that N-glycans with a bisecting GlcNAc on target molecules, such as {beta}1 integrin, play important roles in the regulation of neuritogenesis.

Keywords: bisecting GlcNAc/glycosyltransferase/GnT-III/integrin/neurite formation.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Fiala, P. T. Liu, A. Espinosa-Jeffrey, M. J. Rosenthal, G. Bernard, J. M. Ringman, J. Sayre, L. Zhang, J. Zaghi, S. Dejbakhsh, et al.
Innate immunity and transcription of MGAT-III and Toll-like receptors in Alzheimer's disease patients are improved by bisdemethoxycurcumin
PNAS, July 31, 2007; 104(31): 12849 - 12854.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.