Skip Navigation



Glycobiology Advance Access published online on February 20, 2006
Glycobiology, doi:10.1093/glycob/cwj094
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
16/6/538    most recent
cwj094v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Lukasiewicz, J.
Right arrow Articles by Lugowski, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lukasiewicz, J.
Right arrow Articles by Lugowski, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Article

Structure of the lipid A-inner core region and biological activity of Plesiomonas shigelloides O54 (strain CNCTC 113/92) lipopolysaccharide

Jolanta Lukasiewicz 1 *, Tomasz Niedziela 1, Wojciech Jachymek 1, Lennart Kenne 2, and Czeslaw Lugowski 1

1 Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, 53-114 Wroclaw, Poland
2 Department of Chemistry, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden

* To whom correspondence should be addressed.
Jolanta Lukasiewicz, E-mail: czaja{at}iitd.pan.wroc.pl


  Abstract

Plesiomonas shigelloides is a Gram-negative rod, associated with episodes of intestinal infections and outbreaks of diarrhoea in humans. The extraintestinal infections caused by this bacterium, e.g., endopthalmitis, meningitidis, bacteraemia and septicaemia, usually have gastrointestinal origin and serious course. The lipopolysaccharide (LPS, endotoxin) as virulence factor is important in enteropathogenicity of this bacterium. Lipopolysaccharides of P. shigelloides and especially their lipid A part, i.e. the immunomodulatory centre of LPS, have not been extensively investigated.

The structure of P. shigelloides O54 lipid A was determined by chemical analysis combined with MALDI-TOF mass spectrometry and the intact Kdo-containing core region was investigated by NMR spectroscopy on deacylated LPS. Products from alkaline deacylation of LPS, containing 4-substituted uronic acids are usually very complex and difficult to separate. Since Kdo residues as sialic acids form complexes with serotonin, we used immobilised serotonin for one-step isolation of oligosaccharide containing the intact Kdo region from the reaction mixture by affinity chromatography.

The major form of lipid A was built of {beta}-D-GlcpN4PPEtn-(1->6)-{alpha}-D-GlcpN1P disaccharide substituted with 14:0(3-OH), 12:0(3-OH), 14:0(3-O-14:0) and 12:0(3-O-12:0) acyl groups at N-2, O-3, N-2' and O-3', respectively. This is a novel structure among known lipid A molecules. Analysis of intact Kdo-lipid A region, lipid A and its linkage with the core oligosaccharide completes the structural investigation of P. shigelloides O54 LPS, resolving the entire molecule. Biological activities and observed discrepancy between in vitro and in vivo activity of P. shigelloides and Escherichia coli LPS are discussed.

Keywords: lipid A/lipopolysaccharide/MALDI-TOF mass spectrometry/NMR spectroscopy/Plesiomonas shigelloides.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
J. Lukasiewicz, T. Niedziela, W. Jachymek, L. Kenne, and C. Lugowski
Two Kdo-Heptose Regions Identified in Hafnia alvei 32 Lipopolysaccharide: the Complete Core Structure and Serological Screening of Different Hafnia O Serotypes
J. Bacteriol., January 15, 2009; 191(2): 533 - 544.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.