Glycobiology Advance Access published online on February 15, 2006
Glycobiology, doi:10.1093/glycob/cwj087
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1 National Research Laboratory for Glycobiology and Department of Biological Science, Sungkyunkwan University, Chunchun-Dong, Jangan-Gu, Suwon City, Kyunggi 440-746, Korea; Faculty of Biotechnology, Dong-A University, Saha-Gu, Busan 604-714, Korea
* To whom correspondence should be addressed. The transcriptional regulation mechanisms involved in up-regulation of Fas-induced GD3 synthase gene have not been elucidated yet. The 5’-rapid amplification of cDNA end (5’-RACE) using mRNA prepared from Fas-induced Jurkat T cells revealed the presence of multiple transcription start sites of human GD3 synthase gene, and the 5’-end analysis of the longest its product showed that transcription started from 650 nucleotides upstream of the translational initiation site. Promoter analyses of the 5’-flanking region of the human GD3 synthase gene using luciferase gene reporter system showed strong promoter activity in Fas-induced Jurkat T cells. Deletion study revealed that the region from -1146 to -646 (A of the translational start ATG as position +1) was indispensable for the Fas response. This region lacks apparent TATA and CAAT boxes, but contains putative binding sites for transcription factors c-Ets-1, CREB, AP-1 and NF-
Received August 1, 2005
Revised December 27, 2005
Accepted February 3, 2006
Article
Transcriptional regulation of the human GD3 synthase gene expression in Fas-induced Jurkat T cells: a critical role of transcription factor of NF-
Nam-Young Kang 1,
Sung-Koo Kang 2,
Young-Choon Lee 3,
Hee-Jeong Choi 2,
Young-Sik Lee 4,
Soo-Young Cho 4,
Yong-Sam Kim 5,
Jeong-Heon Ko 5,
and
Cheorl-Ho Kim 2 *
B for regulated expression
2 National Research Laboratory for Glycobiology and Department of Biological Science, Sungkyunkwan University, Chunchun-Dong, Jangan-Gu, Suwon City, Kyunggi 440-746, Korea
3 Faculty of Biotechnology, Dong-A University, Saha-Gu, Busan 604-714, Korea
4 Division of Molecular and Life Science, Hanyang University, Ansan, Kyunggi-Do, Korea
5 Systematic Proteomic Research Center, Korea Research Institute of Bioscience and Biotechnology, Yusong-Gu, Daejon 305-600, Korea
Cheorl-Ho Kim, E-mail: chkimbio{at}daum.net
Abstract 
B. Base-substitution experiment showed that only NF-B binding site of them is required for the maximal expression induced by Fas. Both DNase I footprint and electrophoretic mobility shift assays with the nuclear extract of Fas-induced Jurkat T cells revealed that NF-B was bound specifically to the probe being mediated by its binding site in the promoter sequence. Taken together, these results indicate that NF-B plays an essential role in the transcriptional activity of human GD3 synthase gene in Fas-induced Jurkat T cells. In addition, the translocation of NF-B binding protein to nucleus by Fas-activation is also crucial for the increased expression of the GD3 synthase gene in Fas-activated Jurkat T cells.B/transcriptional regulation.
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