Skip Navigation



Glycobiology Advance Access published online on November 29, 2005
Glycobiology, doi:10.1093/glycob/cwj062
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
16/3/244    most recent
cwj062v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Guérardel, Y.
Right arrow Articles by , K.-H. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guérardel, Y.
Right arrow Articles by , K.-H. K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org.
Received October 9, 2005
Accepted November 14, 2005

Article

Glycomic survey mapping of zebrafish identifies unique sialylation pattern

Yann Guérardel 1, Lan-Yi Chang 2, Emmanuel Maes 3, Chang-Jen Huang 2, and Kay-Hooi Khoo 2 *

1 Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 11529, Taiwan; Unité de Glycobiologie Structurale et Fonctionnelle, USTL, 59655 Villeneuve d’Ascq, France
2 Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 11529, Taiwan; Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan
3 Unité de Glycobiologie Structurale et Fonctionnelle, USTL, 59655 Villeneuve d’Ascq, France

* To whom correspondence should be addressed.
Kay-Hooi Khoo , E-mail: kkhoo{at}gate.sinica.edu.tw


  Abstract

Functional genomics and proteomics studies of the developmental glycobiology of zebrafish are greatly hampered by the current lack of knowledge on its glycosylation profile. To furnish the requisite structural basis for a more insightful functional delineation and genetic manipulation, we have initiated a survey mapping of the possible expression of stage-specific glycoconjugates in zebrafish. High sensitivity mass spectrometry (MS) analysis in conjunction with the usual array of enzymatic and chemical derivatization was employed as the principal method for rapid differential mapping of the glycolipids and sequentially liberated N- and O-glycans from the total extracts. We demonstrated that all developmental stages of the zebrafish under investigation, from fertilized eggs to hatched embryos, synthesize oligo-mannosyl types of N-glycans as well as complex types with additionally {beta}4-galactosylated, Neu5Ac/Neu5Gc mono-sialylated Lewis X termini. A combination of CID-MS/MS and NMR analyses led to the identification of an abundant and unusual mucin-type O-glycosylation, based on a novel sequence Fuc{alpha}1-3GalNAc{beta}1-4(Neu5Ac/Neu5Gc{alpha}2-3)Gal{beta}1-3GalNAc. This core structure may be further oligo-sialylated, but exclusively in the earlier development stages. Similarly, MS and MS/MS analyses of the extracted glycolipid fraction revealed the presence of a heterogeneous family of oligo-sialylated lactosylceramide compounds. In contrast to the O-glycans, these glycolipids only appear in the later development stages, suggesting a complex pattern of regulation for sialyltransferase activities during zebrafish embryogenesis.

Keywords: glycomics/mass spectrometry/sialylation/structure analysis/zebrafish.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
C.-W. Kuo, C.-M. Chen, Y.-C. Lee, S.-T. Chu, and K.-H. Khoo
Glycomics and Proteomics Analyses of Mouse Uterine Luminal Fluid Revealed a Predominance of Lewis Y and X Epitopes on Specific Protein Carriers
Mol. Cell. Proteomics, February 1, 2009; 8(2): 325 - 342.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
H. Yagi, M. Nakagawa, N. Takahashi, S. Kondo, M. Matsubara, and K. Kato
Neural complex-specific expression of xylosyl N-glycan in Ciona intestinalis
Glycobiology, February 1, 2008; 18(2): 145 - 151.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
K. Moriguchi, T. Takemoto, T. Aoki, S.-i. Nakakita, S. Natsuka, and S. Hase
Free Oligosaccharides with Lewis x Structure Expressed in the Segmentation Period of Zebrafish Embryo
J. Biochem., August 1, 2007; 142(2): 213 - 227.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. T. Pilobello, D. E. Slawek, and L. K. Mahal
A ratiometric lectin microarray approach to analysis of the dynamic mammalian glycome
PNAS, July 10, 2007; 104(28): 11534 - 11539.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. Toukach, H. J Joshi, R. Ranzinger, Y. Knirel, and C.-W. von der Lieth
Sharing of worldwide distributed carbohydrate-related digital resources: online connection of the Bacterial Carbohydrate Structure DataBase and GLYCOSCIENCES.de
Nucleic Acids Res., January 12, 2007; 35(suppl_1): D280 - D286.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.