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Glycobiology Advance Access published online on November 10, 2005
Glycobiology, doi:10.1093/glycob/cwj057
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© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Received August 18, 2005
Revised November 4, 2005
Accepted November 6, 2005

Article

CEACAM1, an adhesion molecule of human granulocytes, is fucosylated by fucosyltransferase IX and interacts with DC-SIGN of dendritic cells via LewisX residues

Valentina Bogoevska 1, Andrea Horst 1, Birgit Klampe 1, Lothar Lucka 2, Christoph Wagener 1*, and Peter Nollau 1

1 Institute of Clinical Chemistry, University Clinic Hamburg-Eppendorf, Hamburg, Germany
2 Institute of Biochemistry and Molecular Biology, Charité Campus Benjamin Franklin, Berlin, Germany

* To whom correspondence should be addressed.
Christoph Wagener, E-mail: wagener{at}uke.uni-hamburg.de


  Abstract

The CEA-related cell adhesion molecule 1, CEACAM1, is a glycoprotein expressed on the surface of human granulocytes and lymphocytes, endothelia and many epithelia. CEACAM1 is involved in the regulation of important biological processes such as tumor growth, angiogenesis and modulation of the immune response. CEACAM1, a member of the immunoglobulin superfamily carries several LewisX (LeX) structures as we recently demonstrated by mass spectrometry of native CEACAM1 from human granulocytes. Since LeX residues of pathogens bind to the C-type lectin DC-SIGN expressed on human dendritic cells, we hypothesized that LeX glycans of CEACAM1 are recognized by DC-SIGN. Here, we demonstrate that CEACAM1, the major carrier of LeX-residues in human granulocytes, is specifically recognized by DC-SIGN via LeX residues mediating the internalization of CEACAM1 into immature dendritic cells. Expression studies with CEACAM1 in combination with different fucosyltransferases (FUT) revealed that FUTIX plays a key role in the synthesis of LeX groups of CEACAM1. As LeX groups on CEACAM1 are selectively attached and specifically interact with DC-SIGN, our findings suggest that CEACAM1 participates in immune regulation in physiological conditions and in pathological conditions such as inflammation, autoimmune disease and cancer.

Keywords: carcinoembryonic antigen/CEACAM1/DC-SIGN/fucosyltransferase/LewisX.
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