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Glycobiology Advance Access published online on September 21, 2005
Glycobiology, doi:10.1093/glycob/cwj043
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© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Received August 19, 2005
Revised September 15, 2005
Accepted September 17, 2005

Article

The N-glycosylation defect of cwh8{Delta} yeast cells causes a distinct defect in sphingolipid biosynthesis

Martine Pittet 1, Danièle Uldry 1, Markus Aebi 2, and Andreas Conzelmann 1*

1 Department of Medicine, University of Fribourg, Switzerland
2 Institute of Microbiology, Department of Biology, ETHZ, Zurich, Switzerland

* To whom correspondence should be addressed.
Andreas Conzelmann, E-mail: andreas.conzelmann{at}unifr.ch


  Abstract

CWH8/YGR036c of Saccharomyces cerevisiae has been identified as a dolichylpyrophosphate phosphatase that removes a phosphate from the dolichylpyrophosphate (Dol-PP) generated by the oligosaccharyltransferase while it adds N-glycans to nascent glycoproteins in the ER. Lack of CWH8 was proposed to interrupt the so called dolichol cycle by trapping dolichol in the form of Dol-PP in the ER lumen. Indeed, cwh8{Delta} mutants display a severe deficiency in N-glycosylation. We find that cwh8{Delta} mutants have strongly reduced levels of inositolphosphorylceramide (IPC) whereas its derivative, mannosyl-(inositol-P)2-ceramide (M(IP)2C) is not affected. Microsomes of cwh8{Delta} contain normal ceramide synthase and IPC synthesis activities. Within a large panel of mutants affecting dolichol dependent pathways such as Nor O-glycosylation, or GPI-anchoring, only the mutants having a deficiency of N-glycan addition show the defect in IPC biosynthesis. By mutating genes required for the addition of N-glycans or by treating cells with tunicamycin one can similarly reduce the steady state level of IPC and exactly reproduce the phenotype of cwh8{Delta} cells. Some potential mechanisms by which the lack of N-glycans could lead to the sphingolipid abnormality were further explored.

Keywords: Dolichol/Golgi/unfolded protein response/AUR1.
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