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Glycobiology Advance Access first published online on August 31, 2005
This version published online on September 6, 2005
Glycobiology, doi:10.1093/glycob/cwj033
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© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org
Received July 6, 2005
Revised August 23, 2005
Accepted August 24, 2005

Article

ST6GalNAc I expression in MDA-MB-231 breast cancer cells greatly modifies their O-glycosylation pattern and enhances their tumourigenicity

S. Julien 1, E. Adriaenssens 2, K. Ottenberg 3, A. Furlan 2, G. Courtand 4, A-S. Vercouter Edouart 1, F-G. Hanisch 3, P. Delannoy 5*, and X. Le Bourhis 2

1 Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS n°8576, GDR CNRS n°2590, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d’Ascq, France
2 Laboratoire de Biologie du Développement, ERI8, UPRES EA 1033, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d’Ascq, France
3 Center of Biochemistry, Medical Faculty, and Center for Molecular Medicine Cologne, University of Cologne, Joseph-Stelzmann-Str. 52, D-50931 Cologne
4 Centre Commun de Mesures Imagerie Cellulaire, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d’Ascq, France
5 Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS n°8576, GDR CNRS n°2590, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d’Ascq, France.

* To whom correspondence should be addressed.
P. Delannoy, E-mail: Philippe.Delannoy{at}univ-lille1.fr


  Abstract

Sialyl-Tn is a carbohydrate antigen over-expressed in several epithelial cancers, including breast cancer, and usually associated with poor prognosis. Sialyl-Tn is synthesized by a CMP-Neu5Ac:GalNAc{alpha}2,6-sialyltransferase: ST6GalNAc I, which transfers a sialic acid residue in {alpha}2,6-linkage to the GalNAca1-O-Ser/Thr structure. However, established breast cancer cell lines express neither ST6GalNAc I nor sialyl-Tn. We have previously shown that stable transfection of MDA-MB-231, a human breast cancer cell line, with ST6GalNAc I cDNA induces sialyl-Tn antigen expression. We report here the modifications of the O-glycosylation pattern of a MUC1 related recombinant protein secreted by MDA-MB-231 sialyl-Tn positive cells. We also show that sialyl-Tn expression and concomitant changes in the overall O-glycan profiles induce a decrease of adhesion and an increase of migration of MDA-MB-231. Moreover, STn positive clones exhibit an increased tumour growth in SCID mice. These observations suggest that modification of the O-glycosylation pattern induced by ST6GalNAc I expression are sufficient to enhance the tumourigenicity of MDA-MB-231 breast cancer cells.

Keywords: Breast cancer/cell adhesion/ST6GalNAc I/sialyl-Tn/tumour growth.
The author order has been corrected.
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