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Glycobiology Advance Access published online on August 23, 2005
Glycobiology, doi:10.1093/glycob/cwj029
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© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org
Received June 29, 2005
Revised August 14, 2005
Accepted August 17, 2005

Article

Functional Expression of the CMP-Sialic Acid Transporter in Escherichia coli and its Identification as a Simple Mobile Carrier

Joe Tiralongo 1, Angel Ashikov 2, Françoise Routier 2, Matthias Eckhardt 3, Hans Bakker 2, Rita Gerardy-Schahn 2, and Mark von Itzstein 1*

1 Institute for Glycomics, Griffith University (Gold Coast Campus), PMB 50 Gold Coast Mail Centre, 9726, Australia
2 Abteilung Zelluläre Chemie, Zentrum Biochemie, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany
3 Institut für Physiologische Chemie, Universität Bonn, Nussallee 11, D-53115 Bonn, Germany

* To whom correspondence should be addressed.
Mark von Itzstein, E-mail: m.vonitzstein{at}griffith.edu.au


  Abstract

The architectural conservation of nucleotide sugar transport proteins (NSTs) enabled the theoretical prediction of putative NSTs in diverse gene databases. In the human genome 17 NST sequences have been identified but only 6 have been unequivocally characterized with respect to their transport specificities. Defining transport characteristics of recombinant NSTs has become a major challenge since true zero background systems are widely absent. Production of recombinant NSTs in heterologous systems has developed multi-functionality for some NSTs leading to a novel level of complexity in the field. Assuming that (i) the specificity of NSTs is determined at the primary sequence level and (ii) the proteins are autonomously functional units, final definition of the substrate specificity will depend on the use of isolated transport proteins. Herein we describe the first report of the functional expression of mouse CMP-sialic acid transporter in Escherichia coli, and thus provide significant progress towards the production of transporter proteins in quantities suitable for functional and structural analyses. Recovery of the active NST from inclusion bodies was achieved after solubilization with 8 M urea and stepwise renaturation. After reconstitution into phospholipid vesicles the recombinant protein demonstrated specific transport for CMP-Neu5Ac with no transport of UDP-sugars. Kinetic studies carried out with CMP-Neu5Ac and established CMP-Neu5Ac antagonist’s evaluated natural conformation of the reconstituted protein and clearly demonstrate that the transporter acts as a simple mobile carrier

Keywords: CMP-sialic acid transporter/Escherichia coli/heterologous expression/nucleotide sugar transporter/simple mobile carrier.
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