Skip Navigation



Glycobiology Advance Access published online on August 11, 2005
Glycobiology, doi:10.1093/glycob/cwj028
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
15/12/1368    most recent
cwj028v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Yuita, H.
Right arrow Articles by Irimura, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yuita, H.
Right arrow Articles by Irimura, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org
Received June 8, 2005
Revised August 6, 2005
Accepted August 9, 2005

Article

Retardation of removal of radiation-induced apoptotic cells in developing neural tubes in macrophage Galactose-type C-type lectin-1(MGL1/CD301a)-deficient mouse embryos

Hiroshi Yuita 1, Makoto Tsuiji 1, Yuki Tajika 1, Yoshihisa Matsumoto 2, Kazuya Hirano 2, Norio Suzuki 2, and Tatsuro Irimura 3*

1 Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113 -0033, Japan
2 Department of Radiation Oncology, Faculty of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113 -0033, Japan
3 Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113 -0033, Japan.

* To whom correspondence should be addressed.
Tatsuro Irimura, E-mail: irimura{at}mol.f.u-tokyo.ac.jp


  Abstract

MGL1/CD301a is a C-type lectin that recognizes galactose and N-acetylgalactosamine as monosaccharides and is expressed on limited populations of macrophages and dendritic cells at least in adult mice. In the present study, pregnant mice with Mgl1+/- genotype were mated with Mgl1+/- or Mgl1-/- genotype males and the embryos were used to assess a hypothesis that this molecule plays an important role in the clearance of apoptotic cells. After X-ray irradiation at 1 Gy of developing embryos at 10.5 d.p.c, the number of Mgl1-/- pups was significantly reduced as compared to Mgl1+/+ pups after X-ray irradiation. Distributions of MGL1-positive cells, MGL2-positive cells, and apoptotic cells were histologically examined in irradiated Mgl1+/+ embryos. MGL1-positive cells were detected in the neural tube in which many cells undergo apoptosis, whereas MGL2-positive cells were not observed. Biotinylated recombinant MGL1 bound a significant portion of the apoptotic cells. When Mgl1+/+ and Mgl1-/- embryos were examined for the presence of apoptotic cells, similar numbers of apoptotic cells gave rise but the clearance of these cells was slower in Mgl1-/-embryos than in Mgl1+/+ embryos. These results strongly suggest that MGL1/CD301a is involved in the clearance of apoptotic cells. This process should be essential in the repair and normal development of X-ray irradiated embryos.

Keywords: apoptosis/phagocytosis/lectin/macrophage/galactose.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
K. Saba, K. Denda-Nagai, and T. Irimura
A C-Type Lectin MGL1/CD301a Plays an Anti-Inflammatory Role in Murine Experimental Colitis
Am. J. Pathol., January 1, 2009; 174(1): 144 - 152.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Sakakura, S. Oo-Puthinan, C. Moriyama, T. Kimura, J. Moriya, T. Irimura, and I. Shimada
Carbohydrate Binding Mechanism of the Macrophage Galactose-type C-type Lectin 1 Revealed by Saturation Transfer Experiments
J. Biol. Chem., November 28, 2008; 283(48): 33665 - 33673.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
Y. Sano, K. Usami, R. Izawa, K. Denda-Nagai, N. Higashi, T. Kimura, N. Suzuki, and T. Irimura
Properties of Blocking and Non-blocking Monoclonal Antibodies Specific for Human Macrophage Galactose-type C-type Lectin (MGL/ClecSF10A/CD301)
J. Biochem., January 1, 2007; 141(1): 127 - 136.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
P. J. Coombs, M. E. Taylor, and K. Drickamer
Two categories of mammalian galactose-binding receptors distinguished by glycan array profiling
Glycobiology, August 1, 2006; 16(8): 1C - 7C.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.