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Glycobiology Advance Access published online on July 27, 2005
Glycobiology, doi:10.1093/glycob/cwj022
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© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org
Received March 6, 2005
Revised July 18, 2005
Accepted July 20, 2005

Article

Resolution of the structural isomers of partially methylesterified oligogalacturonides by polysaccharide analysis using carbohydrate gel electrophoresis

Florence Goubet 1, Anna Ström 2, Bernard Quéméner 3, Elaine Stephens 4, Martin A.K. Williams 5, and Paul Dupree 1*

1 Department of Biochemistry, Building O, Downing Site, Cambridge CB2 1QW, United Kingdom
2 Unilever R&D Colworth, Sharnbrook, MK44 1LQ, Bedford, United Kingdom
3 INRA -- UR Biopolymères, Interactions, Assemblages, rue de la Géraudière, 44316 Nantes cedex 03, France
4 Department of Chemistry, Lensfield Road, Cambridge, CB2 1EW, United Kingdom
5 Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand

* To whom correspondence should be addressed.
Paul Dupree, E-mail: p.dupree{at}bioc.cam.ac.uk


  Abstract

Pectins differing in their degree and pattern of methylesterification are important in diverse aspects of plant physiology and also in many industrial applications. Determination of methylesterification fine structure and knowledge of enzyme specificities in modification and fragmentation of pectin are key to understanding the relationship between structure and function. The development of methodologies for the detection, separation and sequencing of different partially methylesterified oligogalacturonides (Me-OGAs) is consequently very important. Polysaccharide Analysis using Carbohydrate gel Electrophoresis (PACE) has been shown to be powerful for the quantitative resolution of species different in degree of polymerization (DP) and / or degree of methylesterification (DM). Mass spectrometry has, to date, been the only tool with which to obtain isomeric information. However, it is not quantitative and the presence of isobaric species makes the interpretation of the fragmentation patterns complicated. Here, we present evidence that Me-OGAs with the same DP and DM but different patterns of methylesterification (structural isomers) can easily be separated and quantified using PACE.

Keywords: Methylesterification/PACE/Pectin Fine Structure.
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