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Glycobiology Advance Access published online on August 3, 2005
Glycobiology, doi:10.1093/glycob/cwj015
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© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org
Received December 13, 2004
Revised July 8, 2005
Accepted July 11, 2005

Article

Murine glycosyltransferases responsible for the expression of globo-series glycolipids: cDNA structures, mRNA expression, and distribution of their products

Yuko Fujii 1, Shin-ichiro Numata 2, Yoko Nakamura 2, Takashi Honda 3, Keiko Furukawa 2, Takeshi Urano 2, Joelle Wiels 4, Makoto Uchikawa 5, Noriyuki Ozaki 3, Sei-ichi Matsuo 6, Yasuo Sugiura 3, and Koichi Furukawa 2*

1 Department of Biochemistry II; Department of Anatomy II, Nagoya University School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065
2 Department of Biochemistry II, Nagoya University School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065
3 Department of Anatomy II, Nagoya University School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065
4 CNRS UMR 1598, Institut Gustave Roussy, Villejuif Cedex 94805, France
5 Tokyo Blood Center, Japanese Red Cross, Hiroo 4-1-31, Shibuya-ku, Tokyo 150-0012, Japan
6 Department of Clinical Immunology, Nagoya University School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065

* To whom correspondence should be addressed.
Koichi Furukawa, E-mail: koichi{at}med.nagoya-u.ac.jp


  Abstract

Biological functions of globo-series glycosphingolipids are not well understood. In this study, murine cDNAs of two glycosyltransferases responsible for the synthesis of globo-series glycolipids and mRNA expression of those genes were analyzed. Distribution of their products was also analyzed. Murine cDNAs for Gb3/CD77 synthase and Gb4 synthase predicted that both of them are type II membrane proteins with 348 and 331 amino acids, respectively. In Northern blotting, Gb3/CD77 synthase gene was mainly expressed in kidney and lung, but also detected in many other tissues. Gb4 synthase was expressed in brain, heart, kidney, liver, skin and testis. In the immunohistological analysis, Gb3/CD77 was mainly expressed in the proximal tubules as revealed with co-incidental expression with angiotensin converting enzyme. In spleen, It was detected in pre-B cells in the peripheral region of the white pulp, as suggested with co-incidental expression with CD10. It was also expressed on the endothelia of the alveolar capillaries in lung and on the sebaceous ducts aside of the hair follicles. Gb4 was also detected mainly in the proximal tubules in kidney and on the endothelia of the alveolar capillaries in lung as Gb3/CD77. But it was also detected on the epithelium of the bronchus, seminiferou tubules and tails of spermatozoa in testis, blood vessels of choroids plexus and endothelial cells in brain, and central and hepatoportal veins in liver. The expression patterns of two genes and their products almost corresponded with some exception. The results would provide essential informatin for the functional studies of globo-series glycolipids.

Keywords: Gb4/Gb3/globoside/globotriaosylceramide/glycosphingolipid.
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