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Glycobiology Advance Access published online on July 6, 2005
Glycobiology, doi:10.1093/glycob/cwj004
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© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org
Received December 17, 2004
Revised June 23, 2005
Accepted June 23, 2005

Article

Molecular Cloning and Functional Expression of a Novel Helicobacter pylori {alpha}-1,4 Fucosyltransferase

Said Rabbani 1, Viktoria Miksa 1, Beat Wipf 2, and Beat Ernst 1*

1 Institute of Molecular Pharmacy, Pharmacenter, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland
2 Hoffmann-La Roche Ltd. CH-4002 Basel, Switzerland


  Abstract

Helicobacter pylori is an important human pathogen which causes both gastric and duodenal ulcers and is associated with gastric cancer and lymphoma. This microorganism synthesizes fucosylated oligosaccharides, predominantly the Type II blood group antigens Lewis X and Y, whereas a small population also expresses the Type I blood group antigens Lewis A and B. These carbohydrate structures are known to mimic host cell antigens and permit the bacteria to escape from the host immune response. Here, we report the cloning and characterization of a novel Helicobacter pylori {alpha}-1,4 fucosyltransferase. In contrast to the family members characterized to date, this enzyme shows exclusively Type I acceptor substrate specificity. The enzyme consisting of 432 amino acids (MW 50’502 Da) was cloned using a PCR-based approach. It exhibits a high degree of identity (75-87%) and similar structural features, e.g. in the heptamer repeat pattern, with other H. pylori fucosyltransferases. The kinetic characterization revealed a very efficient transferase (kcat/Km = 229 mM-1s-1) for the Type I acceptor substrate Gal{beta}-1,3GlcNAc{beta}--Lem (1). Additionally, the enzyme possesses a broad tolerance toward non-natural Type I acceptor substrate analogs and therefore represents a valuable tool for the chemo-enzymatic synthesis of Lewis A, sialyl Lewis A as well as mimetics thereof.

Keywords: Helicobacter pylori/{alpha}-1,4 fucosyltransferase/sialy Lewisa/x and mimetics thereof/enzymatic synthesis.
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