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Glycobiology Advance Access published online on June 15, 2005
Glycobiology, doi:10.1093/glycob/cwi093
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© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org
Received December 28, 2004
Revised June 8, 2005
Accepted June 10, 2005

Article

The size of the C-chain maltosaccharide of glycogen: evidence for the presence of only a single branch

Soledad Bazán 1 and Juan A. Curtino 1*

1 Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), UNC-CONICET, Departamento de Química Biológica Dr. Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000-Córdoba, Argentina

* To whom correspondence should be addressed.
Juan A. Curtino, E-mail: jcurtino{at}dqb.fcq.unc.edu.ar


  Abstract

Glycogen is found in mammals and yeast bound to glycogenin forming proteoglycogen. The branched polysaccharide is joined to the protein through the C-chain, a maltosaccharide considered to be 13 glucose units long and double branched as the other branched glycogen B-chains. We described before the isolation of c-glycogenin, the debranched C-chain bound to glycogenin, from muscle proteoglycogen. In the present work the size of the C-chain is analyzed for the first time. The maltosaccharide moiety of c-glycogenin was auto[14C]glucosylated by a short incubation with UDP-[14C]glucose and the labeled maltosaccharide was released by heating in 2 M NaOH containing 0.1 M NaBH4 and analyzed by HPTLC. The results indicate that the C-chain is about half the size of the B-chains, not long enough to be double branched.

Keywords: C-chain-bound glycogenin/c-glycogenin/glycogenin-bound C-chain/glycogen structure/proteoglycogen.
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