Skip Navigation



Glycobiology Advance Access published online on December 15, 2004
Glycobiology, doi:10.1093/glycob/cwi027
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
15/5/511    most recent
cwi027v2
cwi027v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by F, S.-S.
Right arrow Articles by MA, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by F, S.-S.
Right arrow Articles by MA, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© Oxford University Press 2004; all rights reserved.
Received August 23, 2004
Revised November 2, 2002
Accepted November 17, 2002

Article

Thomsen-Friedenreich antigen expression in gastric carcinomas is associated with MUC1 mucin VNTR polymorphism

Santos-Silva F 1*, Fonseca A 1, Caffrey T 2, Carvalho F 3, Mesquita P 1, Reis C 1, Almeida R 1, David L 3, and Hollingsworth MA 2

1 Institute of Molecular Pathology and Immunology of the University of Porto, IPATIMUP, 4200-465 Porto, Portugal.
2 Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, 68198-6805 Nebraska, USA.
3 Medical Faculty, University of Porto, 4200-465 Porto, Portugal.

* To whom correspondence should be addressed.
Santos-Silva F, E-mail: fsilva{at}ipatimup.pt


  Abstract

Aberrant glycosylation of mucins is a common phenomenon associated with oncogenic transformation. We investigated the association between expression of the tumour associated antigens T, Tn and sialyl-Tn and polymorphism in the length of the MUC1 mucin tandem repeat in a series of gastric carcinomas. We further evaluated the relevance of MUC1 tandem repeat length on the expression of these tumour associated carbohydrate antigens (TACAs) using a gastric carcinoma cell line model expressing recombinant MUC1 constructs carrying 0, 3, 9 and 42 repeats.

Gastric carcinomas showed a high prevalence of Tn and sialyl-Tn antigens, while T antigen was less frequently expressed. The expression of T antigen was significantly higher in gastric carcinomas from patients homozygous for MUC1 large tandem repeat alleles. No significant associations were found for Tn and sialyl-Tn antigens. This novel association was reinforced by the gastric carcinoma cell line model experiments, where de novo expression of T antigen was detected in clones transfected with larger VNTR regions.

Our results indicate that polymorphism in the MUC1 tandem repeat influences the expression of TACAs in gastric cancer cells, and may therefore allow the identification of subgroups of patients that develop more aggressive tumours expressing T-antigen.

Keywords: Gastric carcinoma, MUC1, Mucins, Polymorphism, T-antigen..
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Zhuo, R. Madden, S. A. Elela, and B. Chabot
Modern origin of numerous alternatively spliced human introns from tandem arrays
PNAS, January 16, 2007; 104(3): 882 - 886.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
D. V. Gold, D. E. Modrak, Z. Ying, T. M. Cardillo, R. M. Sharkey, and D. M. Goldenberg
New MUC1 Serum Immunoassay Differentiates Pancreatic Cancer From Pancreatitis
J. Clin. Oncol., January 10, 2006; 24(2): 252 - 258.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.