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Glycobiology Advance Access published online on October 27, 2004
Glycobiology, doi:10.1093/glycob/cwi009
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Received September 19, 2004
Accepted October 20, 2004

ORIGINAL ARTICLES

Nitrogen-fixing bacterium Burkholderia brasiliensis produces a novel yersiniose A-containing O-polysaccharide

Katherine A. Mattos 1, Adriane R. Todeschini 1, Norton Heise 1, Christopher Jones 2, Jose O. Previato 1, and Lucia Mendonça-Previato 1*

1 Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Bloco G, Universidade Federal do Rio de Janeiro, 21944-970, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, RJ, Brasil
2 Laboratory for Molecular Structure, NIBSC, Herts EN6 3QG, UK

* To whom correspondence should be addressed.
Lucia Mendonça-Previato, E-mail: luciamp{at}biof.ufrj.br


  Abstract

Burkholderia brasiliensis, a Gram-negative diazotrophic endophytic bacterium, was first isolated from roots, stems and leaves of rice plant in Brazil. The polysaccharide moiety was released by ammonolysis from the B. brasiliensis lipopolysaccharide (LPS), allowing the unambiguous characterization of a 3,6-dideoxy-4-C-(1-hydroxyethyl)-D-xylo-hexose (Yersiniose A), an uncommon feature for Burkholderia LPS. The complete structure of the yersiniose A-containing O-antigen was identified by sugar and methylation analyses and nuclear magnetic resonance spectroscopy. Our results show that the repeating oligosaccharide motif of LPS O-chain consists of a branched tetrasaccharide with the following structure:

->2-{alpha}-L-Rhap-(1->3)-[{alpha}-YerAp-(1->2)]-{alpha}-L-Rhap-(1->3)-{alpha}-L-Rhap-(1->

Keywords: Nitrogen-fixing bacterium; lipopolysaccharide; yersiniose; Burkholderia.
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