Glycobiology Advance Access published online on October 13, 2004
Glycobiology, doi:10.1093/glycob/cwi005
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1 Cell Regulation Analysis Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Central-4, 1-1-1 Higashi, Tsukuba, 305-8562, Japan
* To whom correspondence should be addressed. E-mail: owlm.nakamura{at}aist.go.jp.
Sialyl-LewisX (sLeX), one of major selectin ligand, is expressed on T- and B-cells in a differentiation or activation-stage specific manner. We have demonstrated before that sLeX expression and core 2
Revised October 7, 2004
Accepted October 8, 2004
ORIGINAL ARTICLES
Not core 2
1,6-N-acetylglucosaminyltransferase-2 and -3 but -1 regulates sialyl-LewisX expression in human precursor-B cell
2 Cell Regulation Analysis Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Central-4, 1-1-1 Higashi, Tsukuba, 305-8562, Japan; Department of Biochemical Oncology, School of Pharmaceutical Sciences, Teikyo University, Sagamiko 199-0195, Japan
3 Cell Regulation Analysis Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Central-4, 1-1-1 Higashi, Tsukuba, 305-8562, Japan; Core Research for Evolution Science and Technology (CREST) of Japan Science and Technology Corporation, Kawaguchi, 332-0012, Japan
4 Department of Biochemical Oncology, School of Pharmaceutical Sciences, Teikyo University, Sagamiko 199-0195, Japan
Abstract 
1,6-N-acetylglucosaminyltransferase (C2GnT) were simultaneously regulated during precursor-B (pre-B) cell differentiation. While three C2GnT family genes, designated C2GnT-1, -2, and -3, were previously identified, their roles have not been fully examined. In the present study, we have investigated the roles of C2GnTs in the regulation of sLeX expression level during pre-B cell differentiation comparing with 
1,3fucosyltransferase-VII (FucT-VII) and 2,3sialyltransferase-IV (ST3Gal-IV). Over-expression of not FucT-VII and ST3Gal-IV but C2GnT-1 blocked the down-regulation of sLeX expression by differentiation induction. Neither C2GnT-2 nor -3 but C2GnT-1 transcript was mainly expressed in B lineage cell lines and bone marrow-derived B lineage cells. Significant down-regulation of C2GnT-1 of the three C2GnTs was observed in KM3 cells during differentiation. The expression of C2GnT-1 correlated well to sLeX expression and differentiation stage. Furthermore, introduction of short interfering RNA against C2GnT-1 markedly reduced C2GnT-1 expression and resulted in down-regulation of sLeX expression. These results suggest that not the other glycosyltransferases but C2GnT-1 regulates sLeX expression level during differentiation of pre-B cells providing the cells with substrate of sLeX structure biosynthesis.
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