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Glycobiology Advance Access published online on May 26, 2004
Glycobiology, doi:10.1093/glycob/cwh101
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Received February 19, 2004
Revised May 25, 2004
Accepted May 25, 2004

ORIGINAL ARTICLES

Effects of galectin-1 on regulation of progesterone production in granulosa cells from pig ovaries in vitro

Hermann Walzel 1*, Josef Brock 1, Ralf Pöhland 2, Jens Vanselow 3, Wolfgang Tomek 2, Falk Schneider 2, Ute Tiemann 2

1 University of Rostock, Institute of Medical Biochemistry and Molecularbiology, Schillingallee 70, 18057 Rostock, Germany
2 Unit of Reproductive Biology, Research Institute of the Biology of Farm Animals, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany
3 Unit of Molecular Biology, Research Institute of the Biology of Farm Animals, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany

* To whom correspondence should be addressed. E-mail: hermann.walzel{at}med.uni-rostock.de.


  Abstract

The detection of galectin-1 (gal-1) in pig granulosa cell lysates by immunoblotting and its cytosolic as well as membrane-associated localization prompted us to study its effects on cell proliferation and regulation of progesterone synthesis. The lectin stimulated the proliferation of granulosa cells from pig ovaries cultured in serum-free medium. Gal-1 inhibited the FSH stimulated progesterone synthesis of granulosa cells. This inhibitory effect was strongly reduced by the disaccharidic competitor lactose at 30 mM. The absence of inhibitory effects on dibutyryl-cAMP (db-cAMP), forskolin, and on pregnenolone-enhanced cellular progesterone synthesis suggests that gal-1interferes the receptor-dependent mechanism of FSH stimulated progesterone production. In FSH stimulated granulosa cells, Western blot analysis revealed the gal-1 mediated suppression of the cytochrome P450 dependent cholesterol side-chain cleavage enzyme (P450SCC) that catalyses the conversion of cholesterol to pregnenolone. In the presence of 30 mM lactose, the gal-1 reduced P450SCC expression was prevented. Strongly reduced mRNA levels were recorded for P450SCC and 3{beta}-hydroxysteroid dehydrogenase/isomerase (3{beta}-HSD) when FSH stimulated granulosa cells were cultured in the presence of gal-1. We conclude that gal-1 exerts its inhibitory effect on steroidogenic activity of granulosa cells by interfering the hormone-receptor interaction resulting in decreased responses to FSH stimulation.

Key words: galectin-1, granulosa cells, pig ovaries, progesterone synthesis


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