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Glycobiology Advance Access published online on May 12, 2004
Glycobiology, doi:10.1093/glycob/cwh092
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Received March 5, 2004
Revised April 23, 2004
Accepted April 27, 2004

ORIGINAL ARTICLES

Cell surface overexpression of Galectin-3 and the presence of its ligand 90k in the blood plasma as determinants in colon neoplastic lesions

Claudia Greco 1, Rosa Vona 2, Maurizio Cosimelli 3, Paola Matarrese 4, Elisabetta Straface 4, Patrizia Scordati 5, Diana Giannarelli 6, Vincenzo Casale 7, Daniela Assisi 7, Marcella Mottolese 5, Anna Moles 8, Walter Malorni 4*

1 Clinical Pathology Service, Polo Oncologico Regina Elena, Rome, Italy
2 Clinical Pathology Service, Polo Oncologico Regina Elena, Rome, Italy; Laboratory of Ultrastructures, Istituto Superiore di Sanitá - viale Regina Elena 299, 00161 Rome, Italy
3 Surgical Department, Polo Oncologico Regina Elena, Rome, Italy
4 Laboratory of Ultrastructures, Istituto Superiore di Sanitá - viale Regina Elena 299, 00161 Rome, Italy; Department of Drug Research and Evaluation, Istituto Superiore di Sanitá - viale Regina Elena 299, 00161 Rome, Italy
5 Pathological Anatomy Service, Polo Oncologico Regina Elena, Rome, Italy
6 Service of Statistics, Polo Oncologico Regina Elena, Rome, Italy
7 Digestive Endoscopy Service, Polo Oncologico Regina Elena, Rome, Italy
8 Institute of Neuroscience, National Research Council, Rome, Italy

* To whom correspondence should be addressed. E-mail: malorni{at}iss.it.


  Abstract

Galectins are a family of beta-galactoside binding molecules involved in cell-extracellular matrix adhesion processes. Specifically, Galectin-3 (Gal-3), one of the members of this family of molecules plays a role in cell adhesion processes as well as in cell survival or apoptosis. Gal-3 was also hypothesized to represent a useful tool in tumor characterization, e.g. in thyroid tumors. We report herein the results obtained by evaluating Gal-3 expression of colon cells from human adenomas and adenocarcinomas with two different methodologies: immunohistochemistry and flow cytometry of living dispersed cells. We found that: i) the expression of Gal-3 was significantly increased on the surface of cells from adenomas with respect to normal mucosal from the same patient, ii) Gal-3 ligand, i.e. 90k molecule, was increased in the blood plasma from patients with both adenomatous and adenocarcinomatous lesions; and, finally, iii) Gal-3 over-expression was not related with the presence of K-ras mutation. Altogether these results clearly indicate that the evaluation of Gal-3 expression (and of its ligand 90k) can be of interest in the characterization of non-malignant and malignant colon cancers.

Key words: Galectin-3, CD44v6, colon, adenoma, carcinoma, K-ras


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