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Glycobiology Advance Access published online on January 21, 2004
Glycobiology, doi:10.1093/glycob/cwh034
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Submitted on September 22, 2003
Revised on October 26, 2003
Accepted on November 14, 2003

© 2004 Oxford University Press

ORIGINAL ARTICLES

Detailed structural features of glycan chains derived from {alpha}1-acid glycoproteins of several different animals--The presence of hypersialylated, O-acetylated sialic acids but not disialy residues

Miyako Nakano 1, Kazuaki Kakehi 1*, Men-Hwei Tsai 2, and Yuan C. Lee 3

1 Faculty of Pharmaceutical Sciences, Kinki University, Kowakae 3-4-1, Higashi-Osaka 577-8502, Japan
2 Ambryx Biotechnology Inc., 5603D Foxwood Drive, Oak Park, CA 91377, USA
3 Biology Department, The Johns Hopkins University, North Charles Street, Baltimore, MD 21218, USA

* To whom correspondence should be addressed. E-mail: k_kakehi{at}phar.kindai.ac.jp.

Abstract

We analyzed carbohydrate chains of human, bovine, sheep and rat {alpha}1-acid glycoprotein (AGP), and found that carbohydrate chains of AGP of different animals showed quite distinct variations. Human AGP is a highly negatively-charged acidic glycoprotein (pKa = 2.6; isoelectic point = 2.7) with a molecular weight of approximately 37000 when examined by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and contains di-, tri- and tetra-antennary carbohydrate chains. Some of the tri- and tetra-antennary carbohydrate chains are substituted with a fucose residue (sialyl Lewis X type structure). In sheep AGP, mono- and disialo-diantennary carbohydrate chains were abundant. Tri- and tetra-sialo triantennary carbohydrate chains were also present as minor oligosaccharides, and some of the sialic acid residues were substituted with N-glycolylneuraminic acid. In rat AGP, very complex mixture of disialo carbohydrate chains were observed. Complexity of the disialooligosaccharides was due to the presence of N, O-acetylneuraminic acids. Triantennary carbohydrate chains carrying N, O-acetylneuraminic acid were also observed as minor component oligosaccharides. We found some novel carbohydrate chains containing both N-acetylneuraminic acid and N-glycolylneuraminic acid in bovine AGP. Interestingly, triantennary carbohydrate chains were hardly detected in bovine AGP but diantennary carbohydrate chains with tri- or tetra-sialyl residues were abundant. Furthermore, the major sialic acid in these carbohydrate chains was N-glycolylneuraminic acid. It should be noted that these sialic acids are attached to multiple sites of the core oligosaccharide, and are not present as disialyl groups.


{alpha}1-acid glycoprotein, N-acetylneuraminic acid, N-glycolylneuraminic acid, HPLC, MALDI-TOF MS
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[Abstract] [Full Text] [PDF]


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