Skip Navigation



Glycobiology Advance Access published online on October 9, 2003
Glycobiology, doi:10.1093/glycob/cwh011
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
14/1/65    most recent
cwh011v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Ikegami-Kawai, M.
Right arrow Articles by Takahashi, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ikegami-Kawai, M.
Right arrow Articles by Takahashi, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Submitted on August 12, 2003
Revised on September 17, 2003
Accepted on September 21, 2003

© 2003 Oxford University Press

ORIGINAL ARTICLES

Enhanced activity of serum and urinary hyaluronidases in streptozotocin-induced diabetic Wistar and GK rats

Mayumi Ikegami-Kawai 1, Ryouzo Okuda 1, Takashi Nemoto 1, Naoya Inada 1, and Tomoko Takahashi 1*

1 Institute of Medicinal Chemistry, Hoshi University, 2-4-41, Ebara, Shinagawaku, Tokyo 142-8501, Japan

* To whom correspondence should be addressed. E-mail: t-tomoko{at}hoshi.ac.jp.

Abstract

Using streptozotocin-induced diabetic Wistar and GK rats as models of type 1 and type 2 diabetes, respectively, we investigated the changes in serum and urinary hyaluronidase activity with the pathological progress. The serum hyaluronidase levels of streptozotocin-induced rats started to increase on the 3rd day after injection and thereafter maintained approx. 3-fold higher levels compared with control rats; those of GK rats were already higher (~2-fold) from the beginning of experiment. The increases of serum hyaluronidase activity in both diabetic rats were similar to those of blood glucose level, indicating that diabetes mellitus was accompanied by enhanced activity of circulating hyaluronidase from the early phase of its development. In zymography, every serum from diabetic and control rats gave two hyaluronidase isomers, a major 73-kDa band (Hyal-1 type) and a minor 132-kDa band, suggesting that the increases in serum hyaluronidase activity were not due to the appearance of novel isomers. The hyaluronidase activity in 24-h urine of streptozotocin-induced rats was 3-, 7-, and 11-fold higher at the 8th, 15th, and 18th week than that of control rats, respectively, while the urinary hyaluronidase activity of GK rats was not significantly different from controls. There was a good correlation between the urinary hyaluronidase activity and the albumin excretion. Thus the increase in urinary hyaluronidase activity may reflect enhanced glomerular permeability in streptozotocin-induced diabetic rats and may be a useful marker for diabetic nephropathy. Relative resistance to SDS-denaturation in zymography of rat serum and urinary hyaluronidases compared with human serum hyaluronidase are also shown.


diabetes, hyaluronidase, serum, urine, zymography
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 FASEB J.Home page
L. Jadin, X. Wu, H. Ding, G. I. Frost, C. Onclinx, B. Triggs-Raine, and B. Flamion
Skeletal and hematological anomalies in HYAL2-deficient mice: a second type of mucopolysaccharidosis IX?
FASEB J, December 1, 2008; 22(12): 4316 - 4326.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Gao, J. R. Koenitzer, J. M. Tobolewski, D. Jiang, J. Liang, P. W. Noble, and T. D. Oury
Extracellular Superoxide Dismutase Inhibits Inflammation by Preventing Oxidative Fragmentation of Hyaluronan
J. Biol. Chem., March 7, 2008; 283(10): 6058 - 6066.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. Nieuwdorp, H. L. Mooij, J. Kroon, B. Atasever, J. A.E. Spaan, C. Ince, F. Holleman, M. Diamant, R. J. Heine, J. B.L. Hoekstra, et al.
Endothelial glycocalyx damage coincides with microalbuminuria in type 1 diabetes.
Diabetes, April 1, 2006; 55(4): 1127 - 1132.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. Nieuwdorp, T. W. van Haeften, M. C.L.G. Gouverneur, H. L. Mooij, M. H.P. van Lieshout, M. Levi, J. C.M. Meijers, F. Holleman, J. B.L. Hoekstra, H. Vink, et al.
Loss of Endothelial Glycocalyx During Acute Hyperglycemia Coincides With Endothelial Dysfunction and Coagulation Activation In Vivo
Diabetes, February 1, 2006; 55(2): 480 - 486.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
M. Ikegami-Kawai, A. Suzuki, I. Karita, and T. Takahashi
Increased Hyaluronidase Activity in the Kidney of Streptozotocin-Induced Diabetic Rats
J. Biochem., December 1, 2003; 134(6): 875 - 880.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.