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Glycobiology Advance Access published online on September 26, 2003
Glycobiology, doi:10.1093/glycob/cwh006
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What's this?

Submitted on May 6, 2003
Revised on August 12, 2003
Accepted on September 8, 2003

© 2003 Oxford University Press

ORIGINAL ARTICLES

Activity and tissue distribution of splice variants of {alpha}6-fucosyltransferase in human embryogenesis

Ivan Martinez-Duncker 1, Jean-Claude Michalski 2, Chantal Bauvy 1, Jean-Jacques Candelier 1, Benoît Mennesson 3, Patrice Codogno 1, Rafael Oriol 1, and Rosella Mollicone 1*

1 Unité de Glycobiologie et Signalisation cellulaire, INSERM U504, IFR 89, GDR CNRS 2590, Université de Paris Sud XI, 16 Ave Paul Vaillant-Couturier, 94807 Villejuif Cedex, France
2 UMR 8576 CNRS, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq, France
3 Hôpital de Pontoise, 95300 Pontoise, France

* To whom correspondence should be addressed. E-mail: mollicone{at}vjf.inserm.fr.

Abstract

The product of the FUT8 gene transfers an {alpha}1-6 fucose on the innermost N-acetylglucosamine of the chitobiose core of N-glycans. Northern blot analysis shows four main transcripts of 3.0, 3.3, 3.9 and 4.2 kb in the embryo. The larger forms around 4 kb decrease in fetus and adult. Fourteen embryo transcripts of FUT8 were cloned. Twelve exons comprising two new 5'UT-exons (A and B) and two new 3'UT-ends (L1 and L2) and the complete genomic organization of the FUT8 gene (330 kb) are described. Transcripts starting with the 5'UT-exon A are always associated with exons C and D. Exon B initiates another series of transcripts associated to exon C and D or directly to exon D. A third series of transcripts starts at exon C. The data suggest an expression of FUT8 regulated by three different promoters, starting transcription in exons A, B or C. The A or C series are better expressed than the B series. After transfection with these cDNA constructs the transcripts with 5'UT-exons A or C have higher expression of FUT8 transcripts and higher {alpha}6-fucosyltransferase activity, whereas the activity of the B series is about two-thirds lower for both parameters, suggesting that exon-B reduces the expression of the transcripts.


FUT8 isoforms, cDNA, {alpha}6-fucosyltransferase, alternative splicing, human embryo, genomic organization
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