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Glycobiology Advance Access published online on August 7, 2003
Glycobiology, doi:10.1093/glycob/cwg106
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Submitted on May 13, 2003
Revised on July 17, 2003
Accepted on July 23, 2003

© 2003 Oxford University Press

ORIGINAL ARTICLES

Occurrence of a nontypable Helicobacter pylori strain lacking Lewis blood group O-antigens and DD-heptoglycan: evidence for the role of the core {alpha}1,6-glucan chain in colonization

Eleonora Altman 1*, Natalia Smirnova 1, Jianjun Li 1, Annie Aubry 1, and Susan M. Logan 1

1 Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, K1A 0R6, Canada

* To whom correspondence should be addressed. E-mail: Eleonora.Altman{at}nrc.ca.

Abstract

The cell envelope of Helicobacter pylori contains lipopolysaccharide (LPS) essential for the physical integrity and functioning of the bacterial cell membrane and the O-chain of which frequently expresses type 2 Lewis x (Lex) and Lewis y (Ley) blood group antigens that mimic human gastric mucosal cell-surface glycoconjugates. This study describes the isolation and structural analysis of the lipopolysaccharide (LPS) from a clinical isolate of H. pylori strain PJ2 that lacks Le antigens but is still capable of colonization. Subsequent composition, methylation and CE-ESMS analyses of LPS revealed its core oligosaccharide structure to be consistent with the previously proposed structural model for H. pylori LPS. In addition it was carrying an unusually long side branch {alpha} 1,6-glucan and was devoid of Le O-chain polysaccharide. Its ability to colonize the mouse stomach was essentially identical to that of DD-heptoglycan- and Le antigen-producing H. pylori strains.


colonization, Helicobacter pylori, Lewis antigen, lipopolysaccharide
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