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Glycobiology Advance Access published online on July 8, 2003
Glycobiology, doi:10.1093/glycob/cwg091
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Submitted on March 3, 2003
Revised on May 8, 2003
Accepted on May 30, 2003

© 2003 Oxford University Press

ORIGINAL ARTICLES

A new kind of carbohydrate array, its use for profiling anti-glycan antibodies, and the discovery of a novel human cellulose-binding antibody

Mikael Schwarz 1*, Larissa Spector 1, Ari Gargir 1, Avraham Shtevi 1, Monica Gortler 1, Rom T Altstock 1, Avinoam A. Dukler 1, and Nir Dotan 1

1 Glycominds Ltd., 1 Yodfat St., Alon Bldg., Lod 71291 Israel

* To whom correspondence should be addressed. E-mail: mikaels{at}glycominds.com.

Abstract

We have used a novel glycan array to analyze the glycan binding antibody repertoire in a pool of affinity purified IgG collected from a healthy human population. The glycan array used is based on mono- and oligosaccharides covalently linked to the surface via a long linker at their reducing ends. They are thus presented to the medium with a well defined orientation and are accessible for specific binding by glycan binding proteins such as antibodies and lectins. A novel anti-cellulose antibody was detected which binds specifically to {beta}4 linked saccharides with a preference for glucopyranose over galactopyranose residues. We also found previously known anti-glycan antibodies against mono- and oligosaccharides that are constituents of commonly occurring bacterial polysaccharides. We propose that this array can facilitate high-throughput screening of glycan binding proteins and the search for biomarkers for personalized medicine.


antibodies, array, cellulose, saccharide, profile
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