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Glycobiology Advance Access published online on June 10, 2003
Glycobiology, doi:10.1093/glycob/cwg087
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Submitted on April 11, 2003
Revised on May 23, 2003
Accepted on May 23, 2003

© 2003 Oxford University Press

ORIGINAL ARTICLES

Cloning of a rat gene encoding the histo-blood group B enzyme: rats have more than one Abo gene

Anne Laure Turcot 1, Antoine Blancher 2, Béatrice Le Moullac-Vaidye 1, Stéphanie Despiau 2, Jézabel Rocher 1, Francis Roubinet 3, Claude Szpirer 4, and Jacques Le Pendu 1*

1 INSERM U419, Institut de Biologie, 9 Quai Moncousu, 44093, Nantes Cedex, France
2 Laboratoire d'Immunogénétique Moléculaire, Université Paul Sabatier, Faculté de Médecine, Bâtiment A2, 133 Route de Narbonne, 31062, Toulouse, Cedex 4, France
3 Laboratoire d'Immunogénétique Moléculaire, Université Paul Sabatier, Faculté de Médecine, Bâtiment A2, 133 Route de Narbonne, 31062, Toulouse, Cedex 4, France; Laboratoire d'Immunohématologie, Etablissement Français du Sang Pyrénées-Méditerranée, Avenue de Grande Bretagne, BP 3210, 31027, Toulouse, Cedex 3, France
4 Université Libre de Bruxelles, IBMM, B-6041 Gosselies, Belgium

* To whom correspondence should be addressed. E-mail: jlependu{at}nantes.inserm.fr.

Abstract

A genomic DNA fragment corresponding to exon 7 of the human ABO gene was amplified from rats of several inbred and outbred strains. Five different sequences were obtained, four of them corresponding to A-type sequences and one to a B-type sequence based on the amino-acids equivalent to residues at positions 266 and 268 of the human enzymes. In rats from inbred strains, a single A-type sequence and the unique B-type sequence were found, whereas some animals of outbred strains presented two or three A-type sequences along with the B-type sequence. The complete coding sequence of the B-type gene was obtained and identification of the exon-intron boundaries, determined by comparison with rat genomic sequences from data banks, revealed that the rat B-type gene structure is identical with that of the mouse Abo gene. Compared with the human ABO gene and the rat A gene, it lacks exon 4. Like the rat A gene (symbol : Abo), the rat B gene (symbol : Abo2) is located on chromosome 3q11-q12. It could be shown by transfection experiments that the B-type cDNA encodes an active B transferase. A transcript of the B gene was found ubiquitously, whereas the B antigen was only detected in a restricted set of tissues. These data indicate that rats have at least two distinct Abo genes, one monomorphic gene encoding a B-specific enzyme, and one or more genes in some cases, encoding an A-specific enzyme.


ABO, galactosaminyltransferase, histo-blood group, antigen, rat
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