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Glycobiology Advance Access published online on April 2, 2003
Glycobiology, doi:10.1093/glycob/cwg069
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Submitted on January 13, 2003
Revised on March 13, 2003
Accepted on March 13, 2003

© 2003 Oxford University Press

ORIGINAL ARTICLES

Structural differences between the alkali-extracted water-soluble cell wall polysaccharides from mycelial and yeast phases of the pathogenic dimorfic fungus Paracoccidioides brasiliensis

Oussama Ahrazem 1, Alicia Prieto 1, Gioconda San-Blas 2, Juan Antonio Leal 1, Jesús Jiménez-Barbero 1, Manuel Bernabé 3*

1 Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006-Madrid, Spain
2 Centro de Microbiología y Biología Celular, IVIC, P.O. Box 21827, Caracas 1020A, Venezuela
3 Instituto de Química Orgánica, CSIC, Juan de la Cierva 3, 28006-Madrid, Spain

* To whom correspondence should be addressed. E-mail: mbernabe{at}iqog.csic.es.

Abstract

Paracoccidioides brasiliensis is a pathogen dimorphic fungus causing paracoccidioidomycosis, the most widespread systemic mycosis in Latin America. We have studied the structure of the alkali-extracted water-soluble cell wall polysaccharides (F1SS) from both mycelial and yeast phases of this fungus by using chemical analysis and NMR spectroscopic techniques. The F1SS polysaccharide from the mycelial phase consists of a trisaccharidic repeating unit of ->6)-[{alpha}-Galf-(1->6)-{alpha}-Manp-(1->2)]-{alpha}-Manp-(1->. The F1SS polysaccharide of the yeast phase maintains a 10% of the structure of the mycelium phase, but the main structure contain a disaccharide repeating unit of ->6)-[-{alpha}-Manp-(1->2)]-{alpha}-Manp-(1->, alternating with a trisaccharide repeating block of ->6)-[{beta}-Galf-(1->6)-{alpha}-Manp-(1->2)]-{alpha}-Manp-(1->.


cell wall polysaccharides, fungi, NMR spectroscopy, Paracoccidioides brasiliensis
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