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Glycobiology Advance Access published online on January 22, 2003
Glycobiology, doi:10.1093/glycob/cwg039
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Submitted on August 5, 2002
Revised on November 26, 2002
Accepted on December 5, 2002

© 2003 Oxford University Press

ORIGINAL ARTICLES

Multiple transcription initiation and alternative splicing in the 5' untranslated region of the Core2 {beta}1-6 N-acetylglucosaminyltransferase I gene

V. Rebecca Falkenberg 1, Karen Alvarez 1, Clara Roman 1, Nevis Fregien 1*

1 Department of Cell Biology and Anatomy, University of Miami School of Medicine, Miami, FL. 33176

* To whom correspondence should be addressed. E-mail: nevis{at}miami.edu.

Abstract

The glycosyltransferase, core2 {beta}1,6 N-acetylglucosaminyltransferase I (C2GnT I), plays an important regulatory role in the synthesis of biologically significant oligosaccharide structures. This gene is expressed in a variety of cell types including lymphocytes and mucin producing cells. The expression pattern of this gene suggests a complex system of regulation. To investigate the molecular regulation of this gene, rapid amplification of cDNA end (RACE) analysis was used to determine the 5' ends of the C2GnT I mRNAs from a number of tissues and to locate potential promoter elements. These experiments identified 5 C2GnT I mRNAs that are different in their 5' untranslated regions. The RACE cDNAs had 4 different 5' terminal sequences (Exons A, B, D and E'), suggesting 4 transcription initiation sites. One mRNA form was the result of alternative exon (Exon C) utilization. These exons are spread across 60 kb of DNA on human chromosome 9 and all splice to the exon (Exon F) that contains the C2GnT I coding region. RT-PCR experiments using primers specific for each of the four 5' end exon sequences revealed that the 5' terminal exons are differentially expressed, suggesting tissue specificity for the different 5' UTRs. These findings are consistent with the presence of multiple, tissue specific promoters for the C2GnT I gene.


Keywords: glycosyltransferase, mRNA, 5' untranslated region, alternative splicing
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