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Glycobiology Advance Access published online on January 3, 2003
Glycobiology, doi:10.1093/glycob/cwg037
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Submitted on September 25, 2002
Revised on November 19, 2002
Accepted on December 4, 2002

© 2003 Oxford University Press

ORIGINAL ARTICLES

Distinct localization of MUC5B glycoforms in the human salivary glands

Enno C.I. Veerman 1*, P.A.M. van den Keijbus 1, K. Nazmi 1, W. Vos 2, J.E. van der Wal 2, E. Bloemena 2, J.G.M. Bolscher 1, A.V. Nieuw Amerongen 1

1 Department of Dental Basic Sciences, Section of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands
2 Department of Pathology, VUMC, Amsterdam, The Netherlands

* To whom correspondence should be addressed. E-mail: ECI.Veerman.obc.acta{at}med.vu.nl.

Abstract

Salivary mucins, encoded by the MUC5B gene, comprise a heterogeneous family of molecules, which are secreted by several glands, including the submandibular, sublingual and palatine glands. Previous studies have shown that heterogeneity in the salivary mucin population is related to its multiglandular origin. In the present study we have addressed the question to what extent the mucin (MUC5B) population from a single human salivary gland is comprised of different glycoforms. Using monoclonal antibodies to defined protein- and sulfated carbohydrate epitopes specific to MUC5B, we have conducted an immunohistochemical study of different salivary gland types, including submandibular, sublingual and labial glands. In all tissues studied we found a mosaic expression pattern of sulfo-Lewis a antigen, recognized by mAb F2, which in salivary glands is exclusively present on MUC5B. On the other hand, mucous acini were uniformly labeled by mAb EU-MUC5Bb, evoked against a peptide-stretch of the tandem repeat region of MUC5B. Double staining with both antibodies confirmed the presence of MUC5B-positive/sulfo-Lewis a positive cells, as well as MUC5B-positive/sulfo-Lewis a negative cells within one glandular unit. These results indicate that one and the same salivary gland synthesizes different MUC5B glycoforms.


Key words: MUC5B, salivary glands, sulfo-Lewis a, immunohistology, glycoforms
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