Glycobiology Advance Access published online on January 3, 2003
Glycobiology, doi:10.1093/glycob/cwg036
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© 2003 Oxford University Press
ORIGINAL ARTICLES
1 Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland Natural anti-NOR antibodies are common in human sera and agglutinate human erythrocytes of a rare NOR phenotype. The NOR phenotype-related antigens are unique neutral glycosphingolipids recognized by these antibodies and Griffonia simplicifolia IB4 isolectin (GSL-IB4). The oligosaccharide chains of NOR glycolipids are terminated by Gal
Revised on November 27, 2002
Accepted on November 28, 2002
Specificity of human anti-NOR antibodies, a distinct species of ‘natural' anti-
-galactosyl antibodies
2 Department of Chemistry, Swedish University of Agricultural Sciences, S-750 07 Uppsala, Sweden
3 Laboratory of Carbohydrate Chemistry, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, v-437 Moscow, Russian Federation
1-4GalNAc
1-3Gal units. To characterize the specificity of anti-NOR antibodies and to compare it with specificities of GSL-IB4 and known anti-Gal1,3Gal antibodies, -galactosylated saccharides and saccharide-polyacrylamide (PAA) conjugates were used. New synthetic oligosaccharides, corresponding to the terminal di- and trisaccharide sequence of NOR glycolipids and the conjugate of the ‘NOR-tri' with HSA were included. These compounds were tested by microtiter plate ELISA and hemagglutination-inhibition. Anti-NOR antibodies reacted most strongly with Gal1-4GalNAc1-3Gal (‘NOR-tri'), and over 100-times less strongly with Gal1-4GalNAc (‘NOR-di'). The antibodies reacted also with Gal1-4Gal and Gal1-4Gal1-4GlcNAc, similarly as with ‘NOR-di', but not with other tested compounds. In turn, anti-Gal1, 3Gal antibodies reacted most strongly with Gal1-3Gal and were very weakly inhibited by the NOR-related oligosaccharides (weaker than by galactose), and ‘NOR-tri' was less active than ‘NOR-di'. GSL-IB4 reacted with all tested -galactosylated saccharides and conjugates, including the similarly active ‘NOR-tri' and ‘NOR-di'. These results showed that anti-NOR represent a new species of anti--galactosyl antibodies with high affinity for the Gal1-4GalNAc1-3Gal sequence present in rare NOR erythrocytes.
Key words: Anti--galactosyl, anti-NOR, Griffonia simplicifolia IB4 lectin, oligosaccharides, polyagglutination
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