Glycobiology Advance Access published online on January 3, 2003
Glycobiology, doi:10.1093/glycob/cwg035
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
© 2003 Oxford University Press
ORIGINAL ARTICLES
1 Department of Bacteriology and Immunology, Haartman Institute and Biomedicum, P.O. Box 63, FIN-00014 University of Helsinki, Helsinki, Finland Actinobacillus actinomycetemcomitans is a Gram-negative coccobacillus, which can cause various forms of severe periodontitis and other non-oral infections in human patients. The serotype a-specific polysaccharide antigen of A. actinomycetemcomitans contains solely 6-deoxy-D-talose and its O-2 acetylated modification. This polysaccharide is synthesized from the donor GDP-6-deoxy-D-talose with the relevant talosylation enzyme(s). In the synthesis of GDP-6-deoxy-D-talose, GDP-D-mannose is first converted by GDP-mannose-4,6-dehydratase (GMD) to GDP-4-keto-6-deoxy-D-mannose, and then reduced to GDP-6-deoxy-D-talose by GDP-6-deoxy-D-talose synthetase (GTS). In this study, we cloned and overexpressed in Escherichia coli the A. actinomycetemcomitans GTS enzyme responsible for the synthesis of GDP-6-deoxy-D-talose. The recombinant A. actinomycetemcomitans GTS enzyme expressed in E. coli converted the GDP-4-keto-6-deoxy-intermediate to a novel GDP-deoxyhexose. The synthesized GDP-deoxyhexose was shown to be GDP-6-deoxy-D-talose by high-pressure liquid chromatography, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and nuclear magnetic resonance spectroscopy. The functional expression of gts provides another enzymatically defined pathway for the synthesis of GDP-deoxyhexoses, which can be used as donors for the corresponding glycosyltransferases.
Revised on November 29, 2002
Accepted on December 1, 2002
Cloning and functional expression of a novel GDP-6-deoxy-D-talose synthetase from Actinobacillus actinomycetemcomitans
2 VTT Biotechnology and Programme for Structural Biology and Biophysics, P.O.Box 65, 00014 Helsinki
3 MediCel, Haartmaninkatu 8, FIN-00290 Helsinki, Finland
4 Department of Bacteriology and Immunology, Haartman Institute and Biomedicum, P.O. Box 63, FIN-00014 University of Helsinki, Helsinki, Finland; HUCH Laboratory Diagnostics, Helsinki University Central Hospital, P.O. Box 401, FIN-00029 HUCH, Helsinki, Finland
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Fruscione, L. Sturla, G. Duncan, J. L. Van Etten, P. Valbuzzi, A. De Flora, E. Di Zanni, and M. Tonetti Differential Role of NADP+ and NADPH in the Activity and Structure of GDP-D-mannose 4,6-Dehydratase from Two Chlorella Viruses J. Biol. Chem., January 4, 2008; 283(1): 184 - 193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Turnock, L. Izquierdo, and M. A. J. Ferguson The de Novo Synthesis of GDP-fucose Is Essential for Flagellar Adhesion and Cell Growth in Trypanosoma brucei J. Biol. Chem., September 28, 2007; 282(39): 28853 - 28863. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. T. T. Thuy, K. Liou, T.-j. Oh, D. H. Kim, D. H. Nam, J. C. Yoo, and J. K. Sohng Biosynthesis of dTDP-6-deoxy-{beta}-D-allose, biochemical characterization of dTDP-4-keto-6-deoxyglucose reductase (GerKI) from Streptomyces sp. KCTC 0041BP Glycobiology, February 1, 2007; 17(2): 119 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maki and R. Renkonen Biosynthesis of 6-deoxyhexose glycans in bacteria Glycobiology, March 1, 2004; 14(3): 1R - 15R. [Abstract] [Full Text] [PDF]
|
|