Skip Navigation



Glycobiology Advance Access published online on December 17, 2002
Glycobiology, doi:10.1093/glycob/cwg029
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
13/5/339    most recent
cwg029v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Zhou, B.
Right arrow Articles by Weigel, P. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhou, B.
Right arrow Articles by Weigel, P. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Submitted on September 6, 2002
Revised on November 6, 2002
Accepted on November 6, 2002

© 2002 Oxford University Press

ORIGINAL ARTICLES

Purification and molecular identification of the human hyaluronan receptor for endocytosis (HARE)

Bin Zhou 1, Carl T. McGary 2, Janet A. Weigel 1, Amit Saxena 1, Paul H. Weigel 1*

1 Department of Biochemistry & Molecular Biology, and The Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190
2 Department of Pathology, St Joseph's Hospital, 69 Exchange St, St Paul, MN 55102

* To whom correspondence should be addressed. E-mail: paul-weigel{at}OUHSC.edu.

Abstract

The clearance of hyaluronan (HA) and chondroitin sulfates from the circulating blood and lymph in the body is mediated by the membrane-bound HA Receptor for Endocytosis (HARE). Previously, we found that two HARE species of ~175 kDa and ~300 kDa are abundant in the sinusoidal endothelial cells in rat liver, spleen, and lymph nodes (Zhou et al., J. Biol. Chem. 275, 37733-37741, 2000). In the present study, immuno-cytochemical analysis of human tissues showed a similar pattern with abundant expression of HARE in the sinusoidal endothelial cells of human liver, spleen, and lymph nodes. The two human HARE proteins were immunoaffinity purified from human spleen. Each protein was recognized in Western blots using several anti-rat HARE monoclonal antibodies, and was able to bind 125I-HA specifically. In non-reducing SDS-PAGE, these two human HARE species migrated at ~190 kDa and ~315 kDa; both proteins are ~15 kDa larger than the corresponding rat HAREs, although the de-N-glycosylated core proteins are essentially the same mass. After reduction, the human 190 kDa HARE gave a single 196 kDa species, which was not seen in the ~315 kDa HARE after reduction. The reduced ~315 kDa HARE yielded two major proteins at ~250 kDa and ~220 kDa. We determined the sequence of the human 190 kDa HARE cDNA based on analysis of internal tryptic peptides, as well as RT-PCR and 5'RACE analyses using human spleen and lymph node cDNA libraries. The human gene that encodes HARE is on chromosome 12.


Key words: hyaluronan binding protein, hyaluronic acid, iso-receptors, receptor mediated endocytosis, recycling receptor.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
E. N. Harris, B. A. Baggenstoss, and P. H. Weigel
Rat and human HARE/stabilin-2 are clearance receptors for high- and low-molecular-weight heparins
Am J Physiol Gastrointest Liver Physiol, June 1, 2009; 296(6): G1191 - G1199.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
E. N. Harris and P. H. Weigel
The ligand-binding profile of HARE: hyaluronan and chondroitin sulfates A, C, and D bind to overlapping sites distinct from the sites for heparin, acetylated low-density lipoprotein, dermatan sulfate, and CS-E
Glycobiology, August 1, 2008; 18(8): 638 - 648.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. S. Pandey, E. N. Harris, J. A. Weigel, and P. H. Weigel
The Cytoplasmic Domain of the Hyaluronan Receptor for Endocytosis (HARE) Contains Multiple Endocytic Motifs Targeting Coated Pit-mediated Internalization
J. Biol. Chem., August 1, 2008; 283(31): 21453 - 21461.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. N. Harris, J. A. Weigel, and P. H. Weigel
The Human Hyaluronan Receptor for Endocytosis (HARE/Stabilin-2) Is a Systemic Clearance Receptor for Heparin
J. Biol. Chem., June 20, 2008; 283(25): 17341 - 17350.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. V. Kyosseva, E. N. Harris, and P. H. Weigel
The Hyaluronan Receptor for Endocytosis Mediates Hyaluronan-dependent Signal Transduction via Extracellular Signal-regulated Kinases
J. Biol. Chem., May 30, 2008; 283(22): 15047 - 15055.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. N. Harris, S. V. Kyosseva, J. A. Weigel, and P. H. Weigel
Expression, Processing, and Glycosaminoglycan Binding Activity of the Recombinant Human 315-kDa Hyaluronic Acid Receptor for Endocytosis (HARE)
J. Biol. Chem., February 2, 2007; 282(5): 2785 - 2797.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. Chai, Q. Chai, C. C. Danielsen, P. Hjorth, J. R. Nyengaard, T. Ledet, Y. Yamaguchi, L. M. Rasmussen, and L. Wogensen
Overexpression of Hyaluronan in the Tunica Media Promotes the Development of Atherosclerosis
Circ. Res., March 18, 2005; 96(5): 583 - 591.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Prevo, S. Banerji, J. Ni, and D. G. Jackson
Rapid Plasma Membrane-Endosomal Trafficking of the Lymph Node Sinus and High Endothelial Venule Scavenger Receptor/Homing Receptor Stabilin-1 (Feel-1/Clever-1)
J. Biol. Chem., December 10, 2004; 279(50): 52580 - 52592.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. N. Harris, J. A. Weigel, and P. H. Weigel
Endocytic Function, Glycosaminoglycan Specificity, and Antibody Sensitivity of the Recombinant Human 190-kDa Hyaluronan Receptor for Endocytosis (HARE)
J. Biol. Chem., August 27, 2004; 279(35): 36201 - 36209.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. A. Weigel and P. H. Weigel
Characterization of the Recombinant Rat 175-kDa Hyaluronan Receptor for Endocytosis (HARE)
J. Biol. Chem., October 31, 2003; 278(44): 42802 - 42811.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
Glyco-Forum
Glycobiology, May 1, 2003; 13(5): 11G - 13G.
[PDF]

Home page
 GlycobiologyHome page
P. H. Weigel
Letter to the Glyco-Forum: HARE raising
Glycobiology, May 1, 2003; 13(5): 12G - 13G.
[Full Text] [PDF]

Home page
 GlycobiologyHome page
B. Smedsrod, S. Johansson, and S. Goerdt
Letter to the Glyco-Forum: Shooting HARE
Glycobiology, May 1, 2003; 13(5): 11G - 12G.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.